FastQCFastQC Report
Thu 26 May 2016
ERR580296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13245837
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195850.1478577759940727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157350.11879204009531447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175100.045.704261
GTATCAA301550.039.8707731
CAACGCA347800.032.7654235
ATCAACG359600.031.7329463
TATCAAC380550.030.9888652
AACGCAG390250.029.9838286
TCAACGC394200.029.1974744
GTACTAG71400.027.0661751
ACGCAGA431050.026.2950367
CGCAGAG452200.024.9608848
GTACATG524600.024.7290081
TAGTACT94950.023.9662934
TACATGG535650.023.8768832
ACATGGG541800.022.857943
CTAGTAC102950.022.7894863
TCTAACG34900.022.4999162
CTAACGC34750.022.3248963
GCAGAGT493150.022.0820799
CTGTGCG105250.021.5237489
GTGGTAT81500.021.1734561
Produced by FastQC (version 0.11.5)