FastQCFastQC Report
Thu 26 May 2016
ERR580296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13245837
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175440.1324491611968349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147480.11134064234672374No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187750.045.1947171
GTATCAA306350.037.151461
CAACGCA356100.030.8170475
ATCAACG367250.029.84373
TATCAAC387350.029.0591562
AACGCAG397100.028.0245256
GTACTAG69150.027.9658551
TCAACGC398900.027.746484
GTACATG527450.025.0745531
ACGCAGA442550.024.7322467
TACATGG543400.023.8796422
CGCAGAG466700.023.3417668
GTGGTAT82250.023.1676451
TAGTACT99650.023.1571584
ACATGGG545700.022.8749473
CATGGGG422450.021.794164
CTAGTAC107000.021.4349273
TAGGCAT100850.021.2969025
GCAGAGT518100.020.6177989
CTGTGCG107150.020.5262749
Produced by FastQC (version 0.11.5)