##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580296_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13245837
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50361981655067	34.0	31.0	34.0	31.0	34.0
2	32.69879351527578	34.0	31.0	34.0	31.0	34.0
3	32.78182548977463	34.0	31.0	34.0	31.0	34.0
4	36.261983217821566	37.0	37.0	37.0	35.0	37.0
5	36.189159280761196	37.0	37.0	37.0	35.0	37.0
6	36.21749089921611	37.0	37.0	37.0	35.0	37.0
7	36.18248714671636	37.0	37.0	37.0	35.0	37.0
8	36.17242232408567	37.0	37.0	37.0	35.0	37.0
9	37.96104292994093	39.0	38.0	39.0	35.0	39.0
10-11	37.90825260797034	39.0	38.0	39.0	35.0	39.0
12-13	37.86911246152282	39.0	38.0	39.0	35.0	39.0
14-15	39.274104158159275	41.0	39.0	41.0	36.0	41.0
16-17	39.253411958791276	41.0	39.0	41.0	36.0	41.0
18-19	39.242403103707225	41.0	39.0	41.0	36.0	41.0
20-21	39.21924012804929	41.0	39.0	41.0	36.0	41.0
22-23	39.14282464747226	40.5	39.0	41.0	35.5	41.0
24-25	39.06018849544955	40.0	39.0	41.0	35.0	41.0
26-27	38.92524896690183	40.0	38.0	41.0	35.0	41.0
28-29	38.81576177481272	40.0	38.0	41.0	35.0	41.0
30-31	38.716999461793165	40.0	38.0	41.0	35.0	41.0
32-33	38.58658354319172	40.0	38.0	41.0	34.0	41.0
34-35	38.56485297984567	40.0	38.0	41.0	34.5	41.0
36-37	38.62323653084361	40.0	38.0	41.0	35.0	41.0
38-39	38.53410260144376	40.0	38.0	41.0	34.0	41.0
40-41	38.41111626241513	40.0	38.0	41.0	34.0	41.0
42-43	38.38363204227864	40.0	38.0	41.0	34.0	41.0
44-45	38.30078952353105	40.0	38.0	41.0	34.0	41.0
46-47	38.12299056677203	40.0	37.5	41.0	33.0	41.0
48-49	37.919966288276086	40.0	37.0	41.0	33.0	41.0
50-51	37.73961803999249	40.0	37.0	41.0	33.0	41.0
52-53	37.545802088610934	40.0	36.0	41.0	33.0	41.0
54-55	37.32463607245054	39.0	36.0	41.0	32.5	41.0
56-57	37.08899392314733	39.0	35.0	41.0	32.0	41.0
58-59	36.83351818386411	39.0	35.0	41.0	32.0	41.0
60-61	36.55241261839475	38.0	35.0	40.0	31.0	41.0
62-63	36.239839392557826	37.5	35.0	40.0	31.0	41.0
64-65	35.925404562958164	37.0	35.0	40.0	31.0	41.0
66-67	35.581298826189695	36.0	35.0	39.0	30.5	41.0
68-69	35.21272932771255	36.0	34.0	39.0	30.0	41.0
70-71	34.881416025276465	35.0	34.0	38.5	30.0	40.0
72-73	34.53013007030057	35.0	34.0	37.0	29.5	39.5
74-75	34.18178451086179	35.0	34.0	37.0	29.0	39.0
76-77	32.973103134214924	34.5	32.0	35.5	27.0	38.0
78-79	33.614457546170925	35.0	33.5	36.0	29.0	37.5
80-81	33.5651497145858	35.0	34.0	36.0	29.0	37.0
82-83	33.38251048235004	35.0	34.0	35.0	29.0	37.0
84-85	33.19187402049414	35.0	33.5	35.0	29.0	36.0
86-87	33.036355460209876	35.0	33.0	35.0	29.0	36.0
88-89	32.89299800382565	35.0	33.0	35.0	29.0	36.0
90-91	32.746274282251846	35.0	33.0	35.0	28.5	35.0
92-93	32.63931214765817	35.0	33.0	35.0	27.5	35.0
94-95	32.54784057813787	35.0	33.0	35.0	27.0	35.0
96-97	32.453121082495585	35.0	33.0	35.0	27.0	35.0
98-99	32.38053639041459	35.0	33.0	35.0	27.0	35.0
100	31.075300262263532	34.0	30.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.16289188164390112
1101	2	0.11445848369181277
1101	3	0.0640671835176434
1101	4	0.039837148482185114
1101	5	0.020442761522133424
1101	6	0.043205311278924796
1101	7	0.013506694770050842
1101	8	0.02935070638022097
1101	9	0.028821187873845133
1101	10-11	0.029986338014566627
1101	12-13	0.024855713974183402
1101	14-15	0.05302566583353041
1101	16-17	0.01656669274880329
1101	18-19	0.05749739120657438
1101	20-21	0.03085313885324581
1101	22-23	0.017859465292616505
1101	24-25	-0.013618253955449688
1101	26-27	-0.028782488855604527
1101	28-29	-0.00853525492563989
1101	30-31	-0.014796236944668806
1101	32-33	-0.013010534295752052
1101	34-35	-0.11057794892136741
1101	36-37	-0.02537963733120563
1101	38-39	0.02931083099016263
1101	40-41	0.07618578132494491
1101	42-43	-0.009435645246973934
1101	44-45	-0.044601369710058236
1101	46-47	0.0028371958243553763
1101	48-49	-0.028909356132317043
1101	50-51	-0.047054865752159
1101	52-53	-0.03757488670511577
1101	54-55	-0.023413481099616718
1101	56-57	-0.047039948385872776
1101	58-59	-0.07901614897175335
1101	60-61	-0.09140337403663779
1101	62-63	-0.07155104601326201
1101	64-65	-0.09210069991254244
1101	66-67	-0.12197331307464765
1101	68-69	-0.07852256379491251
1101	70-71	-0.08731127084080725
1101	72-73	-0.08120071801125306
1101	74-75	-0.09657573356741977
1101	76-77	-0.05979834847443044
1101	78-79	-0.0678815418857397
1101	80-81	-0.07521432432339736
1101	82-83	-0.03432631947425335
1101	84-85	-0.061220312267437293
1101	86-87	-0.1256787903731862
1101	88-89	-0.14810348702780374
1101	90-91	-0.12881826480762726
1101	92-93	-0.18948793926563212
1101	94-95	-0.19207014366280362
1101	96-97	-0.22860266667741058
1101	98-99	-0.25489191053267035
1101	100	-0.2018360369543224
1102	1	0.10499731914493537
1102	2	0.033885583415219855
1102	3	-0.009110080742104287
1102	4	0.001566649869516823
1102	5	0.0018187734751009543
1102	6	-0.007446819237841851
1102	7	-0.0018930348237446992
1102	8	0.005534779611316765
1102	9	-0.05153421933506763
1102	10-11	-0.012547613132745994
1102	12-13	-0.06062756085250243
1102	14-15	-0.027735742963649557
1102	16-17	-0.0897637720587241
1102	18-19	-0.05805059543525459
1102	20-21	-0.06836172860674594
1102	22-23	-0.06587558369344748
1102	24-25	-0.05132900212288405
1102	26-27	-0.10937075312145339
1102	28-29	-0.11739151598008135
1102	30-31	-0.14888082213438736
1102	32-33	-0.16214529108271591
1102	34-35	-0.10602323437775141
1102	36-37	-0.08376279648120999
1102	38-39	-0.10067069735411138
1102	40-41	-0.005102143756317901
1102	42-43	0.00609811063601029
1102	44-45	-0.04278613072484205
1102	46-47	-0.0655171923489064
1102	48-49	-0.0896440773532774
1102	50-51	-0.07644083792873602
1102	52-53	-0.08378660639970548
1102	54-55	-0.08483631290294369
1102	56-57	-0.13051261094558697
1102	58-59	-0.14307014565749654
1102	60-61	-0.1381533781772859
1102	62-63	-0.14237249858800283
1102	64-65	-0.13477129249616127
1102	66-67	-0.1678801502024143
1102	68-69	-0.14766106024223546
1102	70-71	-0.1562602780873803
1102	72-73	-0.16829067384141894
1102	74-75	-0.1722710575972144
1102	76-77	-0.11988451321300175
1102	78-79	-0.0793104974918748
1102	80-81	-0.11632345615558393
1102	82-83	-0.1255861273655441
1102	84-85	-0.15016963769024017
1102	86-87	-0.16431170478900725
1102	88-89	-0.11237240521990088
1102	90-91	-0.1616713772249554
1102	92-93	-0.13951792628579085
1102	94-95	-0.2107627793213851
1102	96-97	-0.2436667857258712
1102	98-99	-0.2726175955486667
1102	100	-0.14032908458574056
1103	1	0.11385399525381956
1103	2	0.07813408251697496
1103	3	0.03541926588835054
1103	4	9.860576572648938E-4
1103	5	-0.007871285684167617
1103	6	0.02959211501875103
1103	7	-0.00517347314793426
1103	8	-0.015942771360066388
1103	9	-0.0038293705563674507
1103	10-11	-0.005561993884427352
1103	12-13	-0.025198757356697854
1103	14-15	-0.03352399144771567
1103	16-17	-0.03175294708146481
1103	18-19	-0.04089026702324361
1103	20-21	-0.04255327750948368
1103	22-23	-0.10038095220845378
1103	24-25	-0.09243400974544613
1103	26-27	-0.07226512318925415
1103	28-29	-0.06493549760668316
1103	30-31	-0.133495395101626
1103	32-33	-0.13922565483975546
1103	34-35	-0.12063636718759341
1103	36-37	-0.04656554874209462
1103	38-39	-0.07643216373740813
1103	40-41	-0.05374103506635919
1103	42-43	-0.13111149293512625
1103	44-45	-0.15900884708056395
1103	46-47	-0.1313113440190321
1103	48-49	-0.10685360459495286
1103	50-51	-0.10447311148545424
1103	52-53	-0.14676028895455318
1103	54-55	-0.10384245613394683
1103	56-57	-0.13286849740933349
1103	58-59	-0.09211262900966943
1103	60-61	-0.09643871982768815
1103	62-63	-0.14590200668894937
1103	64-65	-0.1575931316130692
1103	66-67	-0.13728343395209208
1103	68-69	-0.16637442385329848
1103	70-71	-0.13193894383936566
1103	72-73	-0.14667043728406526
1103	74-75	-0.2109095880260412
1103	76-77	-0.14825844530907517
1103	78-79	-0.12919515862020603
1103	80-81	-0.10440544657208051
1103	82-83	-0.1409991452546464
1103	84-85	-0.10503222585287375
1103	86-87	-0.09619822902975983
1103	88-89	-0.1283276794090824
1103	90-91	-0.14783335328701952
1103	92-93	-0.17420149603748314
1103	94-95	-0.16043295465520657
1103	96-97	-0.17779400348266705
1103	98-99	-0.1192151928430576
1103	100	-0.06539978896133292
1104	1	0.09697637177278295
1104	2	0.05861111763771021
1104	3	0.040296018570970205
1104	4	0.016065808665452153
1104	5	-0.006220506046595631
1104	6	0.014619759169796964
1104	7	-0.0023153453606710173
1104	8	0.0019727053814975193
1104	9	0.0024841939949098446
1104	10-11	-0.0019561354437129808
1104	12-13	-0.021001596207021578
1104	14-15	-0.0013315196032621657
1104	16-17	-0.04091914655563045
1104	18-19	-0.03607308845704438
1104	20-21	-0.04755120443623184
1104	22-23	-0.08885618025961861
1104	24-25	-0.09617001870914521
1104	26-27	-0.07502858877374052
1104	28-29	-0.10026761895835534
1104	30-31	-0.10459773652200965
1104	32-33	-0.1305285156201279
1104	34-35	-0.13328422288237363
1104	36-37	-0.08628378301750672
1104	38-39	-0.06473549549640012
1104	40-41	-0.06604826968490585
1104	42-43	-0.05066501359758746
1104	44-45	-0.09655816175465048
1104	46-47	-0.06667159766902131
1104	48-49	-0.1051306779780461
1104	50-51	-0.1062291967856055
1104	52-53	-0.11011492862112959
1104	54-55	-0.14024254489201127
1104	56-57	-0.0980521008172417
1104	58-59	-0.08385438897474984
1104	60-61	-0.1404157209009469
1104	62-63	-0.09250948806406001
1104	64-65	-0.149055870629617
1104	66-67	-0.14795405997108446
1104	68-69	-0.12879765865853443
1104	70-71	-0.12155555046313538
1104	72-73	-0.12528751676341443
1104	74-75	-0.17438483608890465
1104	76-77	-0.2515414337342321
1104	78-79	-0.11946223355482744
1104	80-81	-0.098333051889405
1104	82-83	-0.0448566928132621
1104	84-85	-0.032745139833707526
1104	86-87	-0.033394714115488
1104	88-89	-0.04740164264956803
1104	90-91	-0.059532069621177186
1104	92-93	-0.12987988535078898
1104	94-95	-0.07223443171680088
1104	96-97	-0.12171395053255907
1104	98-99	-0.11704427759300984
1104	100	-0.12023604764057438
1105	1	0.11443410826601763
1105	2	0.09010917836663879
1105	3	0.024714319300578325
1105	4	0.01289011582512245
1105	5	-0.010135653875948947
1105	6	-0.0016896311727592206
1105	7	0.013068229197727987
1105	8	-0.018628792521838022
1105	9	-0.028595962533401575
1105	10-11	-0.013994309801226734
1105	12-13	-0.01342962028356709
1105	14-15	0.004210512511058084
1105	16-17	-0.0034831621261588452
1105	18-19	-0.029671054753144688
1105	20-21	-0.03168225368730759
1105	22-23	-0.03619111214003112
1105	24-25	-0.04465893827640599
1105	26-27	-0.05795244712936665
1105	28-29	-0.06481596520409028
1105	30-31	-0.08643799499941451
1105	32-33	-0.07449044906123703
1105	34-35	-0.05407135192726287
1105	36-37	-0.06793983890885613
1105	38-39	-0.03341019213592489
1105	40-41	-0.03911681163770453
1105	42-43	-0.004768955798510888
1105	44-45	-0.05064542256490512
1105	46-47	-0.05312848343090337
1105	48-49	-0.09329841130413996
1105	50-51	-0.05702660332278242
1105	52-53	-0.09463250417292102
1105	54-55	-0.0752772980687908
1105	56-57	-0.11372823291740985
1105	58-59	-0.11804521251016098
1105	60-61	-0.0788405229278979
1105	62-63	-0.07929427623486163
1105	64-65	-0.08636141480315729
1105	66-67	-0.11783964609495712
1105	68-69	-0.0840856301458004
1105	70-71	-0.08936588156959147
1105	72-73	-0.10270763723788434
1105	74-75	-0.14362373107579174
1105	76-77	-0.0996075931304432
1105	78-79	-0.045392128829405465
1105	80-81	-0.06195601287454622
1105	82-83	-0.013128301972550105
1105	84-85	-0.013049804592967007
1105	86-87	-0.040571838418742345
1105	88-89	0.01881332744343922
1105	90-91	0.0379339301290571
1105	92-93	-0.013545331744040823
1105	94-95	-0.054147171912958925
1105	96-97	-0.07300013831033425
1105	98-99	-0.11651538217867596
1105	100	0.009780410940162909
1106	1	0.10414327797268186
1106	2	0.08213885953824729
1106	3	0.03982706526400648
1106	4	0.02176953520267233
1106	5	0.04406176380044258
1106	6	0.02400157995999308
1106	7	0.021036104357072816
1106	8	0.03273531614194525
1106	9	0.040753294244701976
1106	10-11	0.009603776332760106
1106	12-13	-0.015963120606521386
1106	14-15	0.02039611268187258
1106	16-17	6.754142935676555E-4
1106	18-19	0.005407639043966128
1106	20-21	-0.003519891974136158
1106	22-23	-0.009873005267692747
1106	24-25	0.017265586361077112
1106	26-27	-0.015498495748516916
1106	28-29	0.005526236690343467
1106	30-31	-0.02050078209747852
1106	32-33	-0.012465118900813366
1106	34-35	-0.01699019102638033
1106	36-37	0.04237913532682569
1106	38-39	0.005267895352936591
1106	40-41	0.023523123553296443
1106	42-43	0.03361269904915076
1106	44-45	0.016722219009743355
1106	46-47	-0.029508949787043548
1106	48-49	0.01647711932481144
1106	50-51	0.0029699276691275145
1106	52-53	-0.010974190224956715
1106	54-55	-0.010372426792677913
1106	56-57	-0.03780342177503826
1106	58-59	-0.07801655255842377
1106	60-61	0.010941898402450079
1106	62-63	-0.005478504555490815
1106	64-65	-2.706955102880215E-4
1106	66-67	0.015012060851006481
1106	68-69	0.026517249448197333
1106	70-71	-0.010870981865927831
1106	72-73	-0.02168422196213271
1106	74-75	-0.07110177082682867
1106	76-77	-0.050377266774539464
1106	78-79	-0.004055579909646667
1106	80-81	0.023213376391048257
1106	82-83	0.054251134897555175
1106	84-85	0.08014316045679948
1106	86-87	0.032272533066937115
1106	88-89	0.05757337247486305
1106	90-91	0.10325753996038856
1106	92-93	0.07465020764705343
1106	94-95	0.09115691027514572
1106	96-97	0.08780715920907056
1106	98-99	0.09307038001463752
1106	100	0.08221862125150636
1107	1	0.08018756965101659
1107	2	0.02348337226097641
1107	3	-0.016979145270198615
1107	4	0.023137297905250875
1107	5	0.00836369240764867
1107	6	0.019924922178859106
1107	7	-0.005024574875029941
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2316	2	-0.013292426589181616
2316	3	-0.014233378383373463
2316	4	-0.14930922824800064
2316	5	-0.0838757473140106
2316	6	-0.06265784870814883
2316	7	-0.03532248658900272
2316	8	-0.0014011155787585494
2316	9	0.009039626322682182
2316	10-11	0.00612323815060023
2316	12-13	0.015614938091218278
2316	14-15	0.04814779576943096
2316	16-17	0.053977762760617054
2316	18-19	0.03166124733177611
2316	20-21	0.03565121766173718
2316	22-23	1.1917970073938022E-4
2316	24-25	-0.007466638574570084
2316	26-27	0.034034194670880424
2316	28-29	0.021295840121084098
2316	30-31	-0.3190690011750874
2316	32-33	-0.10471526562258049
2316	34-35	-0.1721651286592376
2316	36-37	-0.43430900831540953
2316	38-39	-0.28202377709859405
2316	40-41	-0.22567652861977905
2316	42-43	-0.32419688153425597
2316	44-45	-0.17736187153754912
2316	46-47	-0.3045105856940893
2316	48-49	-0.29112827905319705
2316	50-51	-0.26859010601999955
2316	52-53	-0.318179126614865
2316	54-55	-0.18019826186267096
2316	56-57	-0.9638660855244368
2316	58-59	-0.8148926240622458
2316	60-61	-0.4496497102351924
2316	62-63	-0.1396611832178749
2316	64-65	-0.06056253520826971
2316	66-67	-0.007451339215130304
2316	68-69	-0.11285330165326712
2316	70-71	-0.1494337079465211
2316	72-73	-0.0471988295744481
2316	74-75	-0.016197327122050353
2316	76-77	0.019598540972211254
2316	78-79	-0.11132413337715263
2316	80-81	-0.11919725696699857
2316	82-83	-0.20559980682048007
2316	84-85	-0.07151118897108688
2316	86-87	-0.15064233522793558
2316	88-89	-0.1773480667158509
2316	90-91	-0.1466741277155208
2316	92-93	-0.15317190484598342
2316	94-95	-0.10140036684051523
2316	96-97	-0.2780760496422019
2316	98-99	-0.09875925115508721
2316	100	-0.3718348861704115
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	7.0
12	11.0
13	123.0
14	505.0
15	1423.0
16	3455.0
17	6989.0
18	12116.0
19	18617.0
20	27159.0
21	39224.0
22	52109.0
23	58423.0
24	63030.0
25	71450.0
26	85915.0
27	107052.0
28	133039.0
29	164628.0
30	204373.0
31	253857.0
32	322596.0
33	421586.0
34	618242.0
35	953429.0
36	1773015.0
37	3577481.0
38	3692651.0
39	583272.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72751329806578	18.69088149083803	13.21572565981196	24.365879551284227
2	16.423497827791362	19.603676202188854	38.89236044658904	25.08046552343075
3	19.552382103528398	24.218397936882052	29.003170339878587	27.226049619710963
4	13.585762815921571	15.996674531636906	36.82140095320329	33.59616169923823
5	14.45512200550508	37.14069873649532	33.73253389033419	14.671645367665413
6	32.81221111206487	35.387654249406815	17.210184603660757	14.589950034867558
7	28.90679675242601	31.31519317859075	21.143052913423443	18.634957155559796
8	27.10081403953587	34.21262628725768	19.360281459910972	19.32627821329548
9	26.701808320448393	15.434277758987244	19.78220429068419	38.08170962988017
10-11	26.258657679182367	25.069022679028546	26.934164739802025	21.73815490198706
12-13	26.478629389126283	22.563744773732957	27.151799687010953	23.805826150129803
14-15	23.97379880190761	24.059191865742566	24.550256717097596	27.416752615252232
16-17	23.75425284916169	26.869702199017258	25.346173202033604	24.029871749787443
18-19	23.410020815799353	26.558055635833135	26.936759439359527	23.09516410900799
20-21	24.578955502076166	25.714748717877484	26.616203661097636	23.090092118948718
22-23	24.63899822845926	25.347040757667262	26.3534718835146	23.66048913035888
24-25	24.18746357817932	25.48173059958385	26.598723055402235	23.73208276683459
26-27	24.078428779007382	25.74344187413884	26.201855465428576	23.9762738814252
28-29	23.788813146099272	25.82249383417954	26.570403231256112	23.818289788465073
30-31	24.291399232253145	25.652651076905563	26.490720557954482	23.565229132886813
32-33	24.175174080229464	25.75979665414707	26.135146707910888	23.929882557712574
34-35	24.28538740893177	25.843317964695483	26.36580307755057	23.505491548822175
36-37	24.216132226353004	25.54357387287626	26.139899146781104	24.100394753989633
38-39	24.182974606785052	25.679238533885147	26.31973082531488	23.818056034014923
40-41	24.17117185140441	25.215497583811537	26.633249018423438	23.980081546360616
42-43	24.02866876876722	25.2999517440744	26.405311798473775	24.266067688684615
44-45	24.060287376426018	25.557778044783504	26.401009055155694	23.980925523634784
46-47	24.03394700890257	25.486243733982228	26.032372081505134	24.44743717561007
48-49	24.2701027675672	25.516769316949457	26.160528577163717	24.052599338319634
50-51	24.11109171228798	25.520720931571333	26.131976380412393	24.236210975728298
52-53	23.999239868805645	25.592397238461796	26.03611665069344	24.372246242039118
54-55	23.8064858603726	25.52574523310455	26.142854090527166	24.52491481599568
56-57	24.2037813837392	25.457320945770594	25.86395511789103	24.47494255259917
58-59	23.70767523285374	25.631992756022097	26.257808064028936	24.402523947095226
60-61	23.925046387371687	25.7361498000214	25.99830851947892	24.340495293127987
62-63	23.959974547252862	25.55904938813639	25.83939274278113	24.641583321829618
64-65	23.6280076318632	25.485421491708777	26.08179244978543	24.804778426642592
66-67	23.89019367616595	25.500837368174817	25.93041558420576	24.67855337145347
68-69	23.673922614323008	25.42513215598534	26.10686712845718	24.794078101234472
70-71	23.5870821201583	25.24602950002297	26.02409601260966	25.142792367209072
72-73	23.70042479390331	25.408067445186216	26.02286198418449	24.86864577672598
74-75	23.735919442284377	25.57650769665366	25.901763290078545	24.78580957098342
76-77	23.62548859625606	25.466958462306337	25.67586207469297	25.23169086674464
78-79	23.64180199967091	25.418142640936768	25.74685483993041	25.19320051946191
80-81	23.47295492948772	25.797457876766465	25.760906607373308	24.968680586372503
82-83	23.798899969620596	25.395566418860117	26.174019781038783	24.631513830480507
84-85	23.813375391959223	25.46689710227624	25.708776730875478	25.01095077488906
86-87	23.242343178777222	25.778156360776343	25.93991565685859	25.03958480358784
88-89	23.52497988484107	25.184801829587656	25.941418692951608	25.348799592619663
90-91	23.496516805891126	25.335788095811235	25.79591073151285	25.371784366784787
92-93	23.52743618646349	25.329163987624987	25.90325054875921	25.24014927715231
94-95	23.146500408405412	25.278909678721327	26.177699803637744	25.39689010923552
96-97	23.193877725895565	25.23486476755566	26.018721234317717	25.55253627223106
98-99	22.97922300157766	25.314420431116506	25.97492850623692	25.73142806106891
100	23.22478616727456	25.13359803169631	25.745246486037505	25.89636931499163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	836.0
1	604.5
2	546.5
3	775.5
4	662.5
5	579.0
6	971.5
7	1839.0
8	2953.0
9	3638.5
10	3256.5
11	2711.0
12	2808.0
13	3171.5
14	3576.0
15	4064.5
16	4683.5
17	5256.0
18	5788.5
19	6359.5
20	7028.5
21	7750.5
22	8660.5
23	9914.0
24	11357.5
25	12837.0
26	15202.5
27	18574.5
28	22305.5
29	27936.0
30	36459.5
31	47237.5
32	64184.5
33	86085.5
34	110656.5
35	141566.5
36	183547.5
37	235209.5
38	279104.0
39	315640.5
40	338640.0
41	354281.0
42	381586.5
43	418804.0
44	462719.0
45	516364.0
46	565561.0
47	606092.0
48	644610.0
49	659846.5
50	651844.0
51	653086.0
52	653906.0
53	638168.0
54	616897.5
55	566946.0
56	506847.5
57	441387.0
58	378930.5
59	338181.0
60	282779.0
61	221984.0
62	174550.0
63	135447.5
64	98463.0
65	66144.0
66	45905.0
67	33172.5
68	25016.5
69	19262.0
70	14930.5
71	11328.5
72	8277.0
73	5979.5
74	4419.0
75	3088.0
76	1969.5
77	1077.0
78	573.5
79	318.5
80	179.0
81	106.5
82	61.5
83	40.0
84	35.5
85	33.0
86	27.5
87	19.5
88	11.0
89	8.0
90	6.0
91	2.0
92	1.0
93	2.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35426224858421557
2	7.700532627722959E-4
3	0.0019175836151388548
4	0.0013891156897068867
5	0.004642968202009432
6	0.0
7	8.30449597107378E-5
8	3.2463029705106594E-4
9	0.0029971680913784457
10-11	7.172064702290991E-5
12-13	4.529725075131152E-5
14-15	2.3026102465250023E-4
16-17	8.30449597107378E-5
18-19	9.738908911531978E-4
20-21	2.113871701727871E-4
22-23	1.6986469031741823E-4
24-25	0.0
26-27	0.0020044033457455347
28-29	0.010961934681817389
30-31	0.003438816286203734
32-33	0.013589175225393456
34-35	0.010875114951210709
36-37	0.012373698996899933
38-39	0.012452969185714726
40-41	0.013868508271693211
42-43	0.006277443999952588
44-45	0.001347593209851518
46-47	0.005903741681254269
48-49	0.012668131126783458
50-51	0.003582257580249553
52-53	0.005699904052873367
54-55	0.005178985669233285
56-57	1.8496377390118872E-4
58-59	5.737651761832793E-4
60-61	3.3595460973889383E-4
62-63	8.719720769627469E-4
64-65	4.1145002765774637E-4
66-67	0.001283422104620493
68-69	0.0016646739651106988
70-71	8.946207023384027E-4
72-73	0.002680087336119265
74-75	0.007719406482202673
76-77	0.003729473645191316
78-79	0.00156652992181619
80-81	4.4542296572123004E-4
82-83	1.5854037762959034E-4
84-85	0.0015023588165851657
86-87	0.004072977796722095
88-89	0.0017326198412376656
90-91	1.547656067336477E-4
92-93	0.002064799680080617
94-95	0.003170807552591807
96-97	0.0017816918628849202
98-99	0.0020836735345603303
100	0.0024158533734032813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3245837E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.28076920347745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79226470032393	34.589279843815156
2	16.158030143059314	16.248763688119936
3	6.255350686793538	9.435715325084399
4	2.9768312377305373	5.98709457688125
5	1.6505436534259594	4.149530224908757
6	1.0361029486110864	3.1257631938093895
7	0.6895630533252497	2.4270232514844463
8	0.45288092423596615	1.8216960982532935
9	0.3284551782357755	1.4863481109504073
>10	1.5383348726744601	13.343483852279897
>50	0.08368237192855191	2.860286706252315
>100	0.03595212386229621	3.16811053073591
>500	0.0015117680544022522	0.5171389267752652
>1k	4.100182908261272E-4	0.3369199941742804
>5k	4.3159724000115635E-5	0.15246066504443262
>10k+	4.3159724000115635E-5	0.3503850114308461
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17544	0.1324491611968349	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14748	0.11134064234672374	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.5495417918852544E-6	0.0
8	0.0	0.0	0.0	7.5495417918852544E-6	0.0
9	0.0	0.0	0.0	1.5099083583770509E-5	0.0
10-11	0.0	0.0	0.0	6.417110523102465E-5	0.0
12-13	0.0	3.7747708959426272E-6	0.0	6.794587612696728E-5	0.0
14-15	0.0	7.5495417918852544E-6	0.0	6.794587612696728E-5	0.0
16-17	3.7747708959426272E-6	7.5495417918852544E-6	0.0	6.794587612696728E-5	0.0
18-19	7.5495417918852544E-6	7.5495417918852544E-6	0.0	8.30449597107378E-5	0.0
20-21	7.5495417918852544E-6	7.5495417918852544E-6	0.0	1.0191881419045092E-4	0.0
22-23	1.5099083583770509E-5	7.5495417918852544E-6	0.0	2.4158533734032814E-4	0.0
24-25	2.264862537565576E-5	7.5495417918852544E-6	0.0	6.228371978305334E-4	0.0
26-27	2.264862537565576E-5	7.5495417918852544E-6	0.0	0.0015740794636080755	0.0
28-29	2.264862537565576E-5	7.5495417918852544E-6	0.0	0.0059943361827568915	0.0
30-31	3.0198167167541018E-5	7.5495417918852544E-6	0.0	0.020681969738869654	0.0
32-33	3.0198167167541018E-5	7.5495417918852544E-6	0.0	0.05406981831348219	0.0
34-35	3.0198167167541018E-5	7.5495417918852544E-6	0.0	0.10599934152896491	0.0
36-37	3.0198167167541018E-5	7.5495417918852544E-6	0.0	0.19014653434131795	0.0
38-39	3.0198167167541018E-5	7.5495417918852544E-6	0.0	0.3074475399327351	0.0
40-41	3.397293806348364E-5	7.5495417918852544E-6	0.0	0.43210557400034444	0.0
42-43	3.774770895942627E-5	7.5495417918852544E-6	0.0	0.5580885526524295	0.0
44-45	3.774770895942627E-5	7.5495417918852544E-6	0.0	0.7141526805742815	0.0
46-47	3.774770895942627E-5	7.5495417918852544E-6	0.0	0.8987691755530436	0.0
48-49	4.529725075131153E-5	1.1324312687827882E-5	0.0	1.0968200801504655	0.0
50-51	5.284679254319678E-5	1.5099083583770509E-5	0.0	1.2997593130581329	0.0
52-53	5.284679254319678E-5	1.5099083583770509E-5	0.0	1.4961417689195482	0.0
54-55	6.0396334335082035E-5	2.2648625375655765E-5	0.0	1.7136516174855543	0.0
56-57	6.0396334335082035E-5	3.0198167167541018E-5	0.0	1.9682863378131559	0.0
58-59	6.0396334335082035E-5	3.0198167167541018E-5	0.0	2.252692676197057	0.0
60-61	6.0396334335082035E-5	3.0198167167541018E-5	0.0	2.547215400582085	0.0
62-63	6.0396334335082035E-5	3.0198167167541018E-5	0.0	2.844644698556988	0.0
64-65	6.0396334335082035E-5	3.0198167167541018E-5	0.0	3.1476870808541584	0.0
66-67	6.0396334335082035E-5	3.774770895942627E-5	0.0	3.486721903644141	3.7747708959426272E-6
68-69	6.0396334335082035E-5	3.774770895942627E-5	0.0	3.861956779326214	1.1324312687827882E-5
70-71	6.0396334335082035E-5	3.774770895942627E-5	0.0	4.267978686435595	1.5099083583770509E-5
72-73	6.417110523102465E-5	3.774770895942627E-5	0.0	4.676714653819158	1.5099083583770509E-5
74-75	6.794587612696728E-5	3.774770895942627E-5	0.0	5.0813247966134565	1.5099083583770509E-5
76-77	6.794587612696728E-5	4.529725075131152E-5	0.0	5.522848423999179	1.5099083583770509E-5
78-79	6.794587612696728E-5	4.529725075131152E-5	0.0	6.002406642932417	1.5099083583770509E-5
80-81	6.794587612696728E-5	4.529725075131152E-5	0.0	6.5147751704931895	1.5099083583770509E-5
82-83	6.794587612696728E-5	4.529725075131152E-5	0.0	7.035395347232493	2.264862537565576E-5
84-85	6.794587612696728E-5	4.529725075131152E-5	0.0	7.550138205686813	2.264862537565576E-5
86-87	6.794587612696728E-5	4.529725075131152E-5	0.0	8.086008456845724	2.264862537565576E-5
88	6.794587612696728E-5	4.529725075131152E-5	0.0	8.513089810783569	2.264862537565576E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18775	0.0	45.194717	1
GTATCAA	30635	0.0	37.15146	1
CAACGCA	35610	0.0	30.817047	5
ATCAACG	36725	0.0	29.8437	3
TATCAAC	38735	0.0	29.059156	2
AACGCAG	39710	0.0	28.024525	6
GTACTAG	6915	0.0	27.965855	1
TCAACGC	39890	0.0	27.74648	4
GTACATG	52745	0.0	25.074553	1
ACGCAGA	44255	0.0	24.732246	7
TACATGG	54340	0.0	23.879642	2
CGCAGAG	46670	0.0	23.341766	8
GTGGTAT	8225	0.0	23.167645	1
TAGTACT	9965	0.0	23.157158	4
ACATGGG	54570	0.0	22.874947	3
CATGGGG	42245	0.0	21.79416	4
CTAGTAC	10700	0.0	21.434927	3
TAGGCAT	10085	0.0	21.296902	5
GCAGAGT	51810	0.0	20.617798	9
CTGTGCG	10715	0.0	20.526274	9
>>END_MODULE