FastQCFastQC Report
Thu 26 May 2016
ERR580295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6742861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236850.35126039228748746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195220.28952102082483977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114520.16983888589724747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125400.053.3640251
GTATCAA234750.042.4088441
GTGGTAT38750.034.829831
CAACGCA278650.034.819115
ATCAACG281200.034.503113
TATCAAC296250.033.438052
AACGCAG307550.032.1547786
GTACTAG46900.031.3843171
TCAACGC313700.031.1092534
ACGCAGA332550.029.0172237
CGCAGAG338600.028.373678
GTACATG312050.026.5837121
GCAGAGT361950.025.9887779
GCATCGT51750.025.9635948
TACATGG318100.025.687442
TAGGCAT61900.025.5161825
ACATGGG316700.024.9358983
TGGTATC53550.024.7585032
CTAGGCA66400.024.4246164
CTGTGCG64000.023.7867689
Produced by FastQC (version 0.11.5)