FastQCFastQC Report
Thu 26 May 2016
ERR580295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6742861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212890.31572651430898546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188240.27916933183110254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110670.16412914340070187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122650.053.0118641
GTATCAA222050.041.1377451
CAACGCA264750.033.3229685
ATCAACG269550.032.7474943
TATCAAC287150.031.7639082
GTGGTAT38650.031.0176681
AACGCAG294100.030.6668786
TCAACGC300800.029.4701584
GTACTAG49800.028.8875391
ACGCAGA321500.027.3661987
CGCAGAG330000.026.6609158
GTACATG302850.026.3434541
TACATGG308400.025.6267992
TAGGCAT62600.025.0776885
ACATGGG306250.024.878763
GCAGAGT354700.024.577279
AGGGCTA60500.024.3943735
TAGTACT64700.023.9733244
CTAGGCA68800.023.8427094
GGGCTAT65400.023.21216
Produced by FastQC (version 0.11.5)