FastQCFastQC Report
Thu 26 May 2016
ERR580294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4123715
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64820.15718836049533005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51720.12542088868896129No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.045.761591
GTATCAA92250.041.647271
CAACGCA106000.035.3520665
ATCAACG106600.035.2442933
TATCAAC113000.033.583092
TCAACGC113450.033.1991734
AACGCAG116000.032.573186
ACGCAGA133650.028.0250387
GTACATG150200.027.081771
CGCAGAG139550.026.8055218
TACATGG153050.025.624672
GTACTAG15900.024.8435131
ACATGGG156800.024.4105513
GTGGTAT24500.022.8408221
CATGGGG124550.022.8407784
GCAGAGT157650.022.806089
ATGGGGG85600.022.0258645
GTGTAGC26950.020.0664221
GAGTACT73000.018.77218412-13
AGAGTAC141450.018.70791810-11
Produced by FastQC (version 0.11.5)