FastQCFastQC Report
Thu 26 May 2016
ERR580294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4123715
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.14142587448453642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.12018289333768216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53900.042.878671
GTATCAA95450.041.4097021
ATCAACG108200.034.8369833
CAACGCA109350.034.469365
TATCAAC116400.033.2703322
TCAACGC115400.032.7844354
AACGCAG120500.031.706596
ACGCAGA135750.027.6600517
CGCAGAG143300.026.1371448
GTACATG145150.023.9163441
TACATGG144450.023.5886652
ACATGGG148650.022.4800953
GCAGAGT161850.022.3865789
GTGGTAT27250.022.3283791
GTACTAG15050.021.6245251
CATGGGG115950.020.3880124
GTATAAG19650.019.4426961
ATGGGGG79650.019.176765
AGAGTAC143950.019.11373110-11
GAGTACT72850.018.93253512-13
Produced by FastQC (version 0.11.5)