FastQCFastQC Report
Thu 26 May 2016
ERR580293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8337247
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190940.22902044283922499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146260.1754296112373785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.10617413637859115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123600.047.327141
GTATCAA222600.041.6360781
CAACGCA251300.035.5385675
ATCAACG256400.034.738763
TATCAAC279700.032.9769552
GTACTAG59500.032.6393431
AACGCAG287850.031.89256
TCAACGC302000.029.758384
ACGCAGA303300.029.3710617
GTGGTAT40400.028.3998971
CGCAGAG312200.028.3829358
GCATCGT67000.026.50738
GTACATG313450.025.9529171
TAGGCAT80900.025.797245
TACATGG318550.025.2951472
GCAGAGT343550.025.1539
CTAGGCA87800.024.679414
ACATGGG318350.024.2728143
GGGCACC65100.024.11681694
CTGTGCG75500.022.8411059
Produced by FastQC (version 0.11.5)