FastQCFastQC Report
Thu 26 May 2016
ERR580293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8337247
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169070.20278876228568016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135380.1623797399789163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120850.045.985381
GTATCAA219000.042.379121
CAACGCA250800.035.4931955
ATCAACG257600.034.6848073
TATCAAC277900.033.2606622
AACGCAG286150.031.9121486
GTACTAG57900.030.613411
TCAACGC304400.029.6917024
ACGCAGA303000.029.1913367
CGCAGAG312450.028.2330728
GTGGTAT37450.026.9920331
GGGCACC68700.026.47518794
GTACATG309750.025.1361051
GCAGAGT347750.024.987839
CTGTGCG82500.024.7795899
TACATGG314850.024.4594172
TAGTACT84600.024.3890344
TAGGCAT80050.023.8373155
ACATGGG313150.023.6691323
CTAGTAC91650.023.128483
Produced by FastQC (version 0.11.5)