FastQCFastQC Report
Thu 26 May 2016
ERR580292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5995313
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129740.2164023796589102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98310.16397809422126916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.046.397861
GTATCAA184700.040.668871
CAACGCA208400.034.3022655
ATCAACG213350.033.620843
TATCAAC229650.032.299562
AACGCAG237100.031.0433716
GTGGTAT33550.030.5624451
TCAACGC243400.029.623894
TACCCCG5450.028.4770135
ACGCAGA257450.027.7310477
CGCAGAG264000.026.9900658
GCAGAGT287150.024.2907549
GTGATCG30700.024.0364288
GTACTAG43450.023.3823451
GTACATG289300.022.6640971
GCTATTG54900.022.4304318
GTATAAG47950.022.3650741
CGTTAAC12050.022.2490711
TACATGG294500.022.072632
CTGTGCG59100.021.6320329
Produced by FastQC (version 0.11.5)