FastQCFastQC Report
Thu 26 May 2016
ERR580292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5995313
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111010.18516130850883014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85490.14259472357823522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98350.046.6995471
GTATCAA182200.042.365981
CAACGCA214200.034.4705125
ATCAACG221400.033.3710253
TATCAAC234700.032.883652
AACGCAG244300.031.0496396
TCAACGC246400.030.1756614
GTACTAG41450.028.1267491
ACGCAGA267800.027.535247
CGCAGAG272200.026.986558
GTGGTAT36150.025.3582211
TAGGCAT57650.024.131455
GCAGAGT300750.024.0965739
GGGCTAT56300.021.9545156
GCATCGT50900.021.6983458
GTACATG282050.021.3341961
CTAGGCA65650.021.190834
GCTATTG56400.020.9989668
CTATTGA57350.020.9789149
AGGGCTA58700.020.8173895
Produced by FastQC (version 0.11.5)