FastQCFastQC Report
Thu 26 May 2016
ERR580291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8335493
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233570.2802113804186507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191570.22982443869846692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104520.12539150353794312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134850.052.7940941
GTATCAA247600.041.040681
CAACGCA284900.034.448565
ATCAACG288600.034.0708273
TATCAAC311450.032.3315662
AACGCAG315750.031.7675786
GTACTAG57750.031.0229721
TCAACGC327350.030.2116364
GTGGTAT45000.028.9452741
ACGCAGA341850.028.6144337
CGCAGAG344500.028.203058
GCATCGT56900.026.8352648
GTACATG328050.025.6580331
GCAGAGT379350.025.0946469
TAGGCAT74450.024.875085
TACATGG337050.024.6315612
ACATGGG328000.024.4611593
CTAGGCA78750.023.9947554
CTGTGCG67200.023.4237949
TGGTATC56350.022.775312
Produced by FastQC (version 0.11.5)