FastQCFastQC Report
Thu 26 May 2016
ERR580291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8335493
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203200.24377682279860352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176280.21148119253414283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97790.11731759597182795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131250.052.301451
GTATCAA243650.041.7590941
CAACGCA285700.034.104055
ATCAACG286900.034.0279543
TATCAAC311150.032.322612
AACGCAG317850.031.243736
TCAACGC322900.030.4812894
GTACTAG57500.028.9464721
GTGGTAT44100.028.4664211
ACGCAGA343900.028.2347357
CGCAGAG347250.027.8403728
CTGTGCG70550.025.9783469
GCAGAGT379900.025.1361279
GTACATG332700.025.0138571
TAGTACT77450.024.8214174
TAGGCAT73500.024.492235
TACATGG335750.024.296792
ACATGGG331000.023.8709473
GGGCACC69200.023.70266294
GCATCGT66500.022.4744198
Produced by FastQC (version 0.11.5)