FastQCFastQC Report
Thu 26 May 2016
ERR580290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8391420
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154720.1843788059708607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115020.13706857718955792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122150.039.0016821
GTATCAA208900.037.5287861
GGGCACC98900.037.44727394
GTACTAG95150.035.2409931
CAACGCA211950.034.7232065
ATCAACG223950.033.0327763
GCATCGT95600.031.7605618
TAGTACT129900.031.6052724
CTGTGCG130250.031.5756039
TAGGCAT117400.030.7231795
TATCAAC258100.030.1199912
AACGCAG263200.029.359436
GTACTGT144150.029.216296
GTACATG339250.029.0423361
ACTGTGC148400.028.9484868
CTAACGC47750.028.5622223
TCTAACG49150.028.5143012
TACATGG343650.028.3422092
CTAGGCA131300.028.3312784
ACGCAGA262000.028.0583387
Produced by FastQC (version 0.11.5)