FastQCFastQC Report
Thu 26 May 2016
ERR580290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8391420
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134620.16042576822516333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108270.12902464660331625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124450.037.4709361
GTATCAA208550.037.2372471
GTACTAG92750.037.2014541
GGGCACC99000.036.84586794
CAACGCA211950.033.950575
TAGGCAT113550.032.6170275
ATCAACG224200.032.1380583
TAGTACT129300.030.4251144
CTGTGCG128550.030.271949
CTAGGCA129650.029.7266944
TATCAAC259650.029.5064852
AACGCAG265650.028.5724376
GTACTGT142050.028.2884716
ACTGTGC145450.028.0149578
GTACATG332150.027.7112541
GCATCGT115950.027.0358288
TACATGG336550.026.8983042
ACATGGG323350.026.775023
TACTGTG155200.026.7395177
ACGCAGA269800.026.739367
Produced by FastQC (version 0.11.5)