FastQCFastQC Report
Thu 26 May 2016
ERR580289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6447004
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135330.20991145654632756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109820.17034268941046105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.10269886601590443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87750.044.8596421
GTATCAA153050.039.1176261
GTACTAG47050.033.5859071
CAACGCA168550.033.449555
ATCAACG172500.032.689193
TATCAAC191350.031.0918272
AACGCAG195850.029.7096866
GTACATG235700.029.0123081
GCATCGT50350.028.6547158
TCAACGC203300.028.0359784
CTAACGC23050.027.5446263
TACATGG243300.027.4492472
CTGTGCG69100.027.2728359
TAGGCAT61950.027.2491555
ACGCAGA208000.027.1825267
TAGTACT69900.026.978494
ACATGGG240100.026.9329933
TCTAACG23800.026.8748572
TAACGCC23200.026.756944
CGCAGAG215850.026.1703558
Produced by FastQC (version 0.11.5)