FastQCFastQC Report
Thu 26 May 2016
ERR580289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6447004
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117910.18289115378243911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98110.15217921378674498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91900.041.3334121
GTATCAA156200.038.614561
GTACTAG47750.035.344881
CAACGCA172900.033.1903275
ATCAACG179800.031.9168643
TAGGCAT62600.030.6320975
CTAACGC19700.029.8220843
TATCAAC202900.029.6282712
AACGCAG198700.029.5657946
TAACGCC20200.028.8510234
GTACATG230900.028.8096471
CTAGGCA68950.028.6971454
TAGTACT67350.028.4717034
CTGTGCG69300.028.3478729
TCAACGC205700.028.1492824
TCTAACG21550.027.9177572
TACATGG236550.027.658852
GGGCACC63800.026.96717694
ACATGGG233150.026.8914763
ACGCAGA214400.026.6773827
Produced by FastQC (version 0.11.5)