FastQCFastQC Report
Thu 26 May 2016
ERR580288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12468765
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348760.2797069316808842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277700.22271652405029688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156380.1254173929815824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196800.051.0090831
GTATCAA357700.041.997811
CAACGCA428300.034.0068365
ATCAACG432850.033.749293
TATCAAC454700.032.759592
AACGCAG463900.031.7270626
TCAACGC467350.031.317764
ACGCAGA518300.027.9340727
GTGGTAT78200.027.1773071
CGCAGAG532400.027.0348538
GTACTAG64150.026.9727921
GTACATG501300.025.399531
TACATGG508300.024.6706812
GCAGAGT586850.023.9864889
ACATGGG510900.023.7526953
TGGTATC93750.021.817062
CATGGGG389800.021.5906144
GAGTACT301750.021.39150812-13
GCATCGT69200.021.3904328
TAGTACT85700.021.3413854
Produced by FastQC (version 0.11.5)