FastQCFastQC Report
Thu 26 May 2016
ERR580288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12468765
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311150.24954355944634454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261820.20998069977259176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147570.11835173732121826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205400.052.1207921
GTATCAA363800.040.7184141
CAACGCA436750.032.9379355
ATCAACG440800.032.6899263
TATCAAC465200.031.7020912
TCAACGC472100.030.6611124
AACGCAG476600.030.5672386
GTGGTAT86150.027.4810921
GTACTAG60850.026.9714471
ACGCAGA531950.026.821287
CGCAGAG549000.025.8599058
GTACATG501550.023.6300051
GCAGAGT603000.023.1390179
TACATGG506350.023.0462652
TGGTATC97650.022.909172
ACATGGG508850.022.2407843
GAGTACT306950.021.36564412-13
TAGGCAT85100.021.0407585
TAGTACT87000.020.3654164
CATGGGG391250.020.228284
Produced by FastQC (version 0.11.5)