FastQCFastQC Report
Thu 26 May 2016
ERR580287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7743128
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114340.14766642111560083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94680.12227616539465705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80300.042.397231
GTATCAA131400.035.3570561
CAACGCA138550.031.8882565
GTACATG232250.031.4839461
ATCAACG144500.030.7047213
TACATGG235500.030.6399972
ACATGGG231150.029.9305673
GTACTAG44300.028.659891
AACGCAG160850.028.3019036
TATCAAC164250.027.9614962
CATGGGG182600.027.6966064
GAGTACA161100.027.525211
TCAACGC163150.027.3397274
CTAACGC16750.026.0957133
TAACGCC16650.025.9709974
AGTACAT163900.025.6693742
ATGGGGG138500.025.2483275
GCATCGT40800.024.871768
TCTAACG17150.024.6688882
ACGCAGA179050.024.585777
Produced by FastQC (version 0.11.5)