FastQCFastQC Report
Thu 26 May 2016
ERR580287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7743128
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103370.1334990200342807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90470.11683908621941932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84200.043.722051
GTATCAA136700.035.6321531
CAACGCA144800.031.6804875
GTACATG233150.031.3779811
ATCAACG150850.030.5043643
TACATGG234750.030.5033782
ACATGGG235700.029.3942583
AACGCAG164900.028.2721946
GTACTAG44700.027.6629871
TATCAAC173450.027.6218972
TCAACGC169200.027.390224
CATGGGG182450.026.8952544
CTGTGCG52450.026.2516739
CTAACGC19050.026.1538433
TCTAACG18700.026.1484152
ATGGGGG131250.025.1748315
TAACGCC19050.024.9198474
GGCGTCG31500.024.7667398
ACGGGCG31650.024.5030865
TAGTACT56450.024.479494
Produced by FastQC (version 0.11.5)