FastQCFastQC Report
Thu 26 May 2016
ERR580286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358931
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.12606301866214448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45510.10440633265357951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60450.045.5738561
GTATCAA103750.038.96951
CAACGCA115600.033.710975
ATCAACG120700.032.2884183
TATCAAC127200.031.341752
AACGCAG129350.030.9163466
TCAACGC129700.030.3016194
GTACTAG21000.028.4314421
ACGCAGA148550.026.2250847
GTACATG191300.025.4846251
CGCAGAG154800.025.1358978
TACATGG193900.024.6579862
GTGGTAT26100.023.9566251
ACATGGG196750.023.3203243
GCATCGT23650.023.049528
CGTTAAC6950.022.9989811
GCAGAGT175700.021.7747159
GTATAAG24850.021.3779721
CATGGGG157150.021.1497154
TGGTATC28350.021.0603282
Produced by FastQC (version 0.11.5)