FastQCFastQC Report
Thu 26 May 2016
ERR580286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358931
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.10970579713237029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.044.324251
GTATCAA105750.040.9479331
CAACGCA123300.033.427515
ATCAACG124700.032.9772223
TATCAAC132700.032.0866782
AACGCAG136800.030.7804136
TCAACGC135350.030.5903994
ACGCAGA155450.026.3015337
GTACTAG23050.026.1944161
CGCAGAG162200.024.9751998
GTACATG195600.024.7427641
TACATGG200150.023.9503632
GTGGTAT28850.023.7078341
ACATGGG205150.022.4047343
TGGTATC29900.021.5336112
GCAGAGT184000.021.5309059
TAGGCAT32100.020.7898245
CATGGGG164400.020.0965254
ATGGGGG113250.019.2136675
CGTTAAC7700.018.9906861
Produced by FastQC (version 0.11.5)