##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580286_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4358931
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50409377895635	34.0	31.0	34.0	31.0	34.0
2	32.70313960005332	34.0	31.0	34.0	31.0	34.0
3	32.78534507657955	34.0	31.0	34.0	31.0	34.0
4	36.26295988626569	37.0	37.0	37.0	35.0	37.0
5	36.19060751363121	37.0	37.0	37.0	35.0	37.0
6	36.2164035172844	37.0	37.0	37.0	35.0	37.0
7	36.1826911231217	37.0	37.0	37.0	35.0	37.0
8	36.17311744553883	37.0	37.0	37.0	35.0	37.0
9	37.95368841580654	39.0	38.0	39.0	35.0	39.0
10-11	37.90344111893489	39.0	38.0	39.0	35.0	39.0
12-13	37.86318101846531	39.0	38.0	39.0	35.0	39.0
14-15	39.25658871406774	41.0	39.0	41.0	36.0	41.0
16-17	39.23508263838083	41.0	39.0	41.0	36.0	41.0
18-19	39.2229070613873	41.0	39.0	41.0	36.0	41.0
20-21	39.20165689706948	41.0	39.0	41.0	36.0	41.0
22-23	39.13224182718194	40.0	39.0	41.0	35.5	41.0
24-25	39.04814047297376	40.0	38.5	41.0	35.0	41.0
26-27	38.91159185589311	40.0	38.0	41.0	35.0	41.0
28-29	38.802315636563186	40.0	38.0	41.0	35.0	41.0
30-31	38.703178256320186	40.0	38.0	41.0	35.0	41.0
32-33	38.57948221708487	40.0	38.0	41.0	34.0	41.0
34-35	38.56133487774869	40.0	38.0	41.0	34.5	41.0
36-37	38.62425546538819	40.0	38.0	41.0	35.0	41.0
38-39	38.5395724318646	40.0	38.0	41.0	34.0	41.0
40-41	38.422840485430946	40.0	38.0	41.0	34.0	41.0
42-43	38.404300045125744	40.0	38.0	41.0	34.0	41.0
44-45	38.331072572610125	40.0	38.0	41.0	34.0	41.0
46-47	38.16042809578771	40.0	37.5	41.0	33.5	41.0
48-49	37.96840005037933	40.0	37.0	41.0	33.0	41.0
50-51	37.79828827297335	40.0	37.0	41.0	33.0	41.0
52-53	37.60899862833341	40.0	36.0	41.0	33.0	41.0
54-55	37.39840616885195	39.0	36.0	41.0	32.5	41.0
56-57	37.171338569020705	39.0	35.0	41.0	32.0	41.0
58-59	36.927352371487416	39.0	35.0	41.0	32.0	41.0
60-61	36.651126159143146	38.0	35.0	40.0	31.0	41.0
62-63	36.34648460826749	37.5	35.0	40.0	31.0	41.0
64-65	36.03817380912889	37.0	35.0	40.0	31.0	41.0
66-67	35.69643520395253	36.5	35.0	39.0	31.0	41.0
68-69	35.33040669833957	36.0	34.5	39.0	30.0	41.0
70-71	34.99026745318979	35.5	34.0	38.5	30.0	40.0
72-73	34.634820555773885	35.0	34.0	37.5	30.0	39.5
74-75	34.28432372524364	35.0	34.0	37.0	29.0	39.0
76-77	33.07285857472853	34.5	32.5	36.0	28.0	38.5
78-79	33.712616120787416	35.0	33.5	36.0	29.0	37.5
80-81	33.663320318674465	35.0	34.0	36.0	29.0	37.0
82-83	33.47574359401422	35.0	34.0	35.0	29.0	37.0
84-85	33.283975704134804	35.0	34.0	35.0	29.0	36.0
86-87	33.13177669020225	35.0	33.0	35.0	29.0	36.0
88-89	32.98949742494203	35.0	33.0	35.0	29.0	36.0
90-91	32.84443341727594	35.0	33.0	35.0	29.0	35.5
92-93	32.73917859677063	35.0	33.0	35.0	29.0	35.0
94-95	32.6514560565423	35.0	33.0	35.0	28.5	35.0
96-97	32.56282182489239	35.0	33.0	35.0	27.0	35.0
98-99	32.49515156353702	35.0	33.0	35.0	27.0	35.0
100	31.200600101263362	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1535602442581947
1101	2	0.1020064770899296
1101	3	0.07645186853648056
1101	4	0.03465090132736037
1101	5	0.04328283027847135
1101	6	0.09215141923661463
1101	7	0.03801506580558822
1101	8	0.018362071058334095
1101	9	0.023288699978799343
1101	10-11	0.06681234205046138
1101	12-13	0.025492037101429332
1101	14-15	0.041308103863883616
1101	16-17	0.023731213838765086
1101	18-19	0.032791265141213444
1101	20-21	-0.025450410180354766
1101	22-23	-0.07182078991237972
1101	24-25	-0.06617202916150688
1101	26-27	-0.03171240761523819
1101	28-29	-0.04609486928747941
1101	30-31	-0.06429237502663199
1101	32-33	-0.0465456842081764
1101	34-35	-0.15117543648323561
1101	36-37	0.011883165543586927
1101	38-39	0.0417374368030039
1101	40-41	0.04833485333112719
1101	42-43	0.02534908100174249
1101	44-45	-0.01661943310120506
1101	46-47	0.031101651979128064
1101	48-49	-0.045363551044282246
1101	50-51	-0.17331235274115642
1101	52-53	-0.13266422371305708
1101	54-55	-0.1130375103057446
1101	56-57	-0.09971712238626651
1101	58-59	-0.05396748848952626
1101	60-61	-0.112299975842312
1101	62-63	-0.12468379435934906
1101	64-65	-0.08526095093277064
1101	66-67	-0.15022704813604548
1101	68-69	-0.022403002799372018
1101	70-71	-0.12278753119831975
1101	72-73	-0.23606269061463792
1101	74-75	-0.15102197214599045
1101	76-77	-0.18431926092608109
1101	78-79	-0.0761344246816904
1101	80-81	-0.12860362944791603
1101	82-83	-0.11697616011740308
1101	84-85	-0.08540905836346013
1101	86-87	-0.03724769359269686
1101	88-89	-0.12341039204143556
1101	90-91	-0.18593059001717194
1101	92-93	-0.1713978761230237
1101	94-95	-0.18080881916416303
1101	96-97	-0.12949879735776193
1101	98-99	-0.12576599360148322
1101	100	-0.11029837800354514
1102	1	0.08908857091503108
1102	2	0.01531979794095406
1102	3	0.026069829883162754
1102	4	-0.007285838415981516
1102	5	0.010713208201678981
1102	6	0.020038554484024473
1102	7	-0.04083411449055774
1102	8	-0.05286171027588438
1102	9	-0.0405215731128834
1102	10-11	-0.00736695399857723
1102	12-13	-0.11094223742516363
1102	14-15	-0.07344515352451708
1102	16-17	-0.08906545343189265
1102	18-19	-0.0634353334715172
1102	20-21	-0.036822802628961426
1102	22-23	-0.11426947687756694
1102	24-25	-0.12031311480861007
1102	26-27	-0.14073057494353947
1102	28-29	-0.15293171787843818
1102	30-31	-0.1296118204188872
1102	32-33	-0.20039442655151873
1102	34-35	-0.10266820189505665
1102	36-37	-0.0832223261754379
1102	38-39	-0.1380643104587449
1102	40-41	-0.05756273683028468
1102	42-43	-0.023005144641473407
1102	44-45	-0.06385004179285403
1102	46-47	-0.048994922497819005
1102	48-49	-0.06911934019209554
1102	50-51	-0.013275088840210003
1102	52-53	-0.03590559741544297
1102	54-55	-0.15836758683897756
1102	56-57	-0.1321454491215519
1102	58-59	-0.10028438381984728
1102	60-61	-0.10112543347684522
1102	62-63	-0.08497993412564853
1102	64-65	-0.10745749735459498
1102	66-67	-0.1533772230687731
1102	68-69	-0.11496716550028196
1102	70-71	-0.16750159769341622
1102	72-73	-0.13663668332971213
1102	74-75	-0.09031810485275571
1102	76-77	-0.19153418698527247
1102	78-79	-0.10604997430723273
1102	80-81	-0.12495450207214986
1102	82-83	-0.0750393942276304
1102	84-85	-0.11876872115809789
1102	86-87	-0.11005398798118193
1102	88-89	-0.02212393508487054
1102	90-91	-0.11617043389156834
1102	92-93	-0.11458135638625322
1102	94-95	-0.08895691153324492
1102	96-97	-0.14977514950110304
1102	98-99	-0.16326356525121355
1102	100	-0.13347729832829458
1103	1	0.06388406416132852
1103	2	0.008468071117079035
1103	3	-0.0028657914179675004
1103	4	0.017303354020064887
1103	5	-0.0075351189321395395
1103	6	0.02783150717888816
1103	7	0.03141902803744756
1103	8	-8.429102371394492E-4
1103	9	-0.04477453320156144
1103	10-11	-0.03000996122648303
1103	12-13	-0.05187078146093427
1103	14-15	-0.014192382779413037
1103	16-17	-0.11243740727557139
1103	18-19	-0.08183882295855938
1103	20-21	-0.08899843877630076
1103	22-23	-0.10973085382171632
1103	24-25	-0.11120393658273997
1103	26-27	-0.1473444454379731
1103	28-29	-0.1129479250608938
1103	30-31	-0.2348617424323507
1103	32-33	-0.264228553419251
1103	34-35	-0.2633648010961309
1103	36-37	-0.19695395121825499
1103	38-39	-0.23791982352648944
1103	40-41	-0.2546062938031355
1103	42-43	-0.28768311620061127
1103	44-45	-0.22209438015840277
1103	46-47	-0.310172077787378
1103	48-49	-0.30202925177388096
1103	50-51	-0.3013789667012574
1103	52-53	-0.2473583051436492
1103	54-55	-0.22720182250080967
1103	56-57	-0.23615919818503528
1103	58-59	-0.25415776234317633
1103	60-61	-0.20635725801037808
1103	62-63	-0.32307464294556354
1103	64-65	-0.2672149628788887
1103	66-67	-0.25275911567185005
1103	68-69	-0.28310099207536865
1103	70-71	-0.2177993849260602
1103	72-73	-0.24456385551374638
1103	74-75	-0.3773903217480168
1103	76-77	-0.4218578065398404
1103	78-79	-0.2957428684415788
1103	80-81	-0.21613065206238957
1103	82-83	-0.23570646733789147
1103	84-85	-0.2150614890076028
1103	86-87	-0.18388116667193088
1103	88-89	-0.20204003182039543
1103	90-91	-0.2383712872557453
1103	92-93	-0.272550963499377
1103	94-95	-0.23179992834339913
1103	96-97	-0.22312232653484187
1103	98-99	-0.21997748447284948
1103	100	-0.09583992747159797
1104	1	0.1075285990309709
1104	2	0.038038616259306934
1104	3	-0.010575093648718337
1104	4	-0.006260680675268304
1104	5	0.013588909765523738
1104	6	0.03247944386786372
1104	7	0.014545286832941429
1104	8	-0.016182831811470066
1104	9	-0.040251548074245136
1104	10-11	0.01675201054471387
1104	12-13	-0.00861607977938661
1104	14-15	-0.01329508417990155
1104	16-17	-0.04133437462274969
1104	18-19	-0.04776481963808976
1104	20-21	-0.03598549065765155
1104	22-23	-0.056098375993556715
1104	24-25	-0.012362676719668286
1104	26-27	-0.028260928023279064
1104	28-29	-0.07793579168916409
1104	30-31	-0.13158305527905867
1104	32-33	-0.09812571106922263
1104	34-35	-0.0638610470074994
1104	36-37	-0.004755393462502866
1104	38-39	-0.0467551223112892
1104	40-41	-0.11305392383275148
1104	42-43	-0.09960981595486373
1104	44-45	-0.09203169839047831
1104	46-47	-0.0698032030018112
1104	48-49	-0.008606963384835353
1104	50-51	0.026525351980225764
1104	52-53	-0.04586306365462889
1104	54-55	-0.16748992838778776
1104	56-57	-0.14425329894471872
1104	58-59	-0.15598747668195045
1104	60-61	-0.18476458253498862
1104	62-63	-0.1877689100006208
1104	64-65	-0.17404681760131524
1104	66-67	-0.19343969493682778
1104	68-69	-0.1873930886747459
1104	70-71	-0.15475852503345067
1104	72-73	-0.2032578753842813
1104	74-75	-0.23094745744415945
1104	76-77	-0.1976371174053142
1104	78-79	-0.07210009335000223
1104	80-81	-0.10341022757422991
1104	82-83	-0.04236902053641245
1104	84-85	-0.08211046562816904
1104	86-87	-0.1020771306632966
1104	88-89	-0.057242136527506204
1104	90-91	-0.04223411511766528
1104	92-93	-0.06832296861956877
1104	94-95	-0.08567908878601571
1104	96-97	-0.12931274581372776
1104	98-99	-0.04408654324149097
1104	100	-0.08263244284168536
1105	1	0.13056569746447622
1105	2	0.11136312886308986
1105	3	0.048109136728747615
1105	4	0.01836470442177074
1105	5	0.02331577721717082
1105	6	0.039374977459523564
1105	7	0.021681469919073493
1105	8	0.010329337879738887
1105	9	0.0640848169799284
1105	10-11	-0.001737969411962581
1105	12-13	0.04138486893496207
1105	14-15	0.08935789138168104
1105	16-17	0.08284099591961791
1105	18-19	0.08151579109330953
1105	20-21	0.04223022292131873
1105	22-23	0.03346326706863323
1105	24-25	0.048293953937502465
1105	26-27	0.05934266117137099
1105	28-29	0.0035478957460171046
1105	30-31	-0.004058028888600518
1105	32-33	0.024943828767284515
1105	34-35	0.028291743207695674
1105	36-37	0.05927842889837365
1105	38-39	0.03712811825629103
1105	40-41	0.024564729094677773
1105	42-43	0.04726042167262534
1105	44-45	0.07688342129331005
1105	46-47	0.10658871136028836
1105	48-49	0.08229398667160126
1105	50-51	0.07743243859876969
1105	52-53	0.07891971546754917
1105	54-55	0.11455775816069291
1105	56-57	0.07071148986915432
1105	58-59	0.06511873350964237
1105	60-61	0.14374025752797337
1105	62-63	0.16120571489854996
1105	64-65	0.09952746141048152
1105	66-67	0.09383332515416498
1105	68-69	0.1058128670300178
1105	70-71	0.08027411768210868
1105	72-73	-0.034094238818703104
1105	74-75	-0.023740838102135342
1105	76-77	0.07960035597862714
1105	78-79	0.13115200689532003
1105	80-81	0.04543079057675925
1105	82-83	0.03256460416793061
1105	84-85	0.04977934096830694
1105	86-87	0.07812537588630875
1105	88-89	0.1285756020244051
1105	90-91	0.11580179626593434
1105	92-93	0.08240182600691526
1105	94-95	0.07788273661444123
1105	96-97	0.1445065791737008
1105	98-99	0.06512968449438006
1105	100	0.16038883751543764
1106	1	0.11079403433299717
1106	2	0.10503015592374254
1106	3	0.001280371277921688
1106	4	-0.008156532495199542
1106	5	-0.015453954628597444
1106	6	0.005902842185413704
1106	7	0.03346335789850485
1106	8	0.056768205743566114
1106	9	0.01551608916670233
1106	10-11	0.008263301243118804
1106	12-13	-0.05664457055812733
1106	14-15	-0.018601910112089115
1106	16-17	-0.02329330777214267
1106	18-19	-0.03767910664396368
1106	20-21	-0.028567617955161495
1106	22-23	-0.02191804222016458
1106	24-25	-0.025516904677786556
1106	26-27	-0.10875117968190295
1106	28-29	-0.032552285281077786
1106	30-31	-0.012752515050642899
1106	32-33	-0.0349056008555948
1106	34-35	-0.06621804171155787
1106	36-37	-0.0628105970672479
1106	38-39	-0.12195274756338392
1106	40-41	-0.07946776807160205
1106	42-43	-0.07797991391033321
1106	44-45	-0.11361100355124165
1106	46-47	-0.16199019369506829
1106	48-49	-0.0681825539901908
1106	50-51	-0.09125247439485662
1106	52-53	-0.06620184661910145
1106	54-55	-0.15713462689490854
1106	56-57	-0.21153938384998128
1106	58-59	-0.202844696426979
1106	60-61	-0.09850608363796454
1106	62-63	-0.16208601785742616
1106	64-65	-0.17829007121937224
1106	66-67	-0.12140701992995417
1106	68-69	-0.11161632020228751
1106	70-71	-0.1138668272202139
1106	72-73	-0.11623356392333051
1106	74-75	-0.13743203911322865
1106	76-77	-0.2016730984464843
1106	78-79	-0.07874873649040381
1106	80-81	-0.05983380696736873
1106	82-83	-0.03260815016673746
1106	84-85	-0.021052404046166373
1106	86-87	-0.003077809962526601
1106	88-89	-0.05532869349486447
1106	90-91	0.06842281366110825
1106	92-93	0.03824366150293912
1106	94-95	0.04984981519638865
1106	96-97	-0.06806601531145873
1106	98-99	-0.026757705361553974
1106	100	0.023110032637305267
1107	1	0.10350991744264348
1107	2	0.06461643191929767
1107	3	0.04778549827315004
1107	4	0.01764923714615918
1107	5	0.025526572617529553
1107	6	0.015604332788733188
1107	7	-0.019418791482380016
1107	8	0.055989393270365895
1107	9	0.0011484518027629065
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2316	16-17	-9.984236645976807E-5
2316	18-19	-0.043435012520575356
2316	20-21	0.0034979039996159145
2316	22-23	-0.026320777291715558
2316	24-25	-0.00831314259241367
2316	26-27	0.016091416806105485
2316	28-29	0.027503065250947145
2316	30-31	-0.2291637824949433
2316	32-33	-0.05237643030569217
2316	34-35	-0.16581240012010312
2316	36-37	-0.40877349353865355
2316	38-39	-0.2807780655774934
2316	40-41	-0.2512314766643442
2316	42-43	-0.41158618942299086
2316	44-45	-0.21387702322775937
2316	46-47	-0.3426816260035821
2316	48-49	-0.21993916624148113
2316	50-51	-0.20592907283529627
2316	52-53	-0.2957306360433236
2316	54-55	-0.15517321488128033
2316	56-57	-0.9594679063698095
2316	58-59	-0.6995830102217013
2316	60-61	-0.3289019165396141
2316	62-63	-0.10127030178931307
2316	64-65	-0.02924347912793479
2316	66-67	-0.024943293573073788
2316	68-69	-0.09409028922470952
2316	70-71	-0.12064862938827758
2316	72-73	-0.02029491561340535
2316	74-75	-0.01081398362718744
2316	76-77	0.029906389308486325
2316	78-79	-0.0511607778270573
2316	80-81	-0.08827400178150668
2316	82-83	-0.284736016554362
2316	84-85	-0.08356584064740957
2316	86-87	-0.13695585579586123
2316	88-89	-0.10926575444074871
2316	90-91	-0.061380516637029814
2316	92-93	-0.10721543663308353
2316	94-95	-0.0929640797568041
2316	96-97	-0.26712051162837014
2316	98-99	-0.03498352454313647
2316	100	-0.31022938504827735
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	12.0
13	37.0
14	129.0
15	394.0
16	903.0
17	1938.0
18	3418.0
19	5280.0
20	8068.0
21	11954.0
22	16449.0
23	18496.0
24	20009.0
25	22514.0
26	27365.0
27	34384.0
28	42747.0
29	53168.0
30	66141.0
31	82502.0
32	104908.0
33	138152.0
34	206973.0
35	314810.0
36	567520.0
37	1164709.0
38	1252501.0
39	193423.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3462135038492	19.09867662636999	13.747784266084691	23.807325603696118
2	16.1827110249019	20.06942110461228	38.77386637240316	24.97400149808266
3	19.481135635802936	24.95522304509906	29.405640554213324	26.158000764884683
4	13.80953680980821	16.657906769148674	37.33643443646864	32.196121984574475
5	14.508300883303091	37.49193863474255	33.37310005866357	14.62666042329079
6	31.76930306994995	35.66904362560454	17.964955169054065	14.596698135391453
7	28.61682964251761	31.91468177496852	21.076971218611547	18.391517363902317
8	27.55793351516741	33.760549347212006	19.459824786833614	19.221692350786974
9	26.85856644587975	16.08129571352502	19.868344589099348	37.19179325149588
10-11	26.213612613009435	25.317578083418674	27.009381664482646	21.459427639089242
12-13	26.806840259022373	22.618953258811196	27.121286802479094	23.452919679687337
14-15	24.182922792131357	24.138278716223578	24.726635330165426	26.952163161479643
16-17	23.928408503425903	27.069194002053948	25.25569661605825	23.746700878461905
18-19	23.466693401310906	26.692309289397976	26.960485454032607	22.880511855258515
20-21	24.676757623076085	25.801084420118336	26.60623808262575	22.915919874179828
22-23	24.66414310867932	25.38257712624099	26.37927929478025	23.574000470299445
24-25	24.239016401039613	25.52994071252791	26.59622278948669	23.634820096945788
26-27	23.980059862167664	25.879622051384466	26.26549248751935	23.874825598928524
28-29	23.741055723218622	26.01736147319565	26.543111090002657	23.69847171358307
30-31	24.422012097407876	25.63379473867144	26.548109163448068	23.39608400047261
32-33	24.3063652284047	25.80430222373147	26.145247230732434	23.7440853171314
34-35	24.311842828600614	25.963977577360037	26.420768199351514	23.30341139468784
36-37	24.246658853373177	25.762553276627298	26.17319859837958	23.81758927161994
38-39	24.20783857899886	25.836914164673573	26.28947786247772	23.665769393849843
40-41	24.311051501075013	25.26985988773443	26.642049419656832	23.77703919153372
42-43	24.042006541238816	25.426263999589782	26.3932596630451	24.138469796126305
44-45	24.082050145553747	25.71966456451682	26.359750036190565	23.83853525373887
46-47	24.01864143638081	25.531623471984734	25.97348920326053	24.476245888373928
48-49	24.286135314532046	25.5578603154871	26.21544537908253	23.94055899089832
50-51	24.211785240528645	25.589133549938587	26.18820108590168	24.010880123631097
52-53	24.121266738691382	25.682068622009968	26.02336390598182	24.17330073331683
54-55	23.89721937894389	25.544870335227966	26.24145090285096	24.316459382977186
56-57	24.30774447303077	25.54563095481302	25.84341103642266	24.303213535733555
58-59	23.86629640594823	25.732531099227664	26.24870366115569	24.152468833668415
60-61	24.1156999330223	25.804526747319027	26.108925997269733	23.970847322388938
62-63	24.180222797930433	25.608100241288906	25.95186954454496	24.2598074162357
64-65	23.774631427605918	25.519194808430452	26.211534848252306	24.494638915711324
66-67	24.008122750204556	25.576270539017177	26.086701170269304	24.328905540508966
68-69	23.701463986724942	25.579435956861108	26.246825138092582	24.472274918321364
70-71	23.717126936961904	25.394355165812932	26.157474108075267	24.731043789149894
72-73	23.700936208970237	25.674482842477953	26.082783057961446	24.541797890590363
74-75	23.831438414072455	25.77246973501721	26.00397835536074	24.392113495549587
76-77	23.65397313085345	25.634997983368642	25.75310513050289	24.957923755275015
78-79	23.635579652599255	25.59425849417158	25.958883941235506	24.811277911993663
80-81	23.576354854972262	25.998854070658762	25.810377944473473	24.614413129895507
82-83	23.9167188489171	25.604785495678232	26.282946366434906	24.195549288969758
84-85	23.924343053498283	25.72281809037433	25.821375981492405	24.531462874634983
86-87	23.319249746142116	26.068118799524083	26.06783201926796	24.544799435065837
88-89	23.51812912400305	25.462801998643453	26.176636005337638	24.84243287201586
90-91	23.670935574315802	25.486097879945763	26.014806644258222	24.82815990148022
92-93	23.642360860183974	25.57852040740244	26.07819520582908	24.70092352658451
94-95	23.287040951018295	25.579987338201594	26.245907542258667	24.887064168521448
96-97	23.23661857024854	25.602369524887138	26.154418993915478	25.006592910948843
98-99	23.00565850283281	25.64452663249625	26.09559863252561	25.254216232145332
100	23.224132725585868	25.510963038688384	25.899049010422065	25.365855225303687
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	57.5
2	76.5
3	128.0
4	114.0
5	103.0
6	188.5
7	376.0
8	610.0
9	752.5
10	729.5
11	652.5
12	692.0
13	817.5
14	920.0
15	1051.5
16	1200.5
17	1410.0
18	1644.5
19	1812.5
20	2021.0
21	2312.0
22	2684.5
23	3177.0
24	3728.5
25	4413.5
26	5442.0
27	6640.5
28	8099.0
29	10384.5
30	13547.0
31	17161.5
32	22525.5
33	30006.5
34	38339.5
35	47841.5
36	60499.5
37	76304.0
38	90454.5
39	103442.0
40	113894.5
41	121756.0
42	132081.0
43	144998.5
44	159775.0
45	178919.0
46	194952.5
47	207463.0
48	218142.0
49	221590.0
50	219159.0
51	217200.5
52	213998.5
53	206826.5
54	198854.5
55	182888.5
56	163844.5
57	141015.5
58	117643.0
59	101614.5
60	83438.5
61	65164.0
62	51435.5
63	39680.0
64	28687.5
65	19411.0
66	13779.5
67	10308.0
68	7758.0
69	5735.5
70	4198.0
71	3161.5
72	2297.5
73	1634.0
74	1228.0
75	832.0
76	528.5
77	316.0
78	166.0
79	77.5
80	37.5
81	18.5
82	7.0
83	5.0
84	5.5
85	4.5
86	2.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35701413947594035
2	5.964765214223396E-4
3	0.0023859060856893585
4	0.0011929530428446793
5	0.004083570031276017
6	0.0
7	6.882421401026994E-5
8	2.752968560410798E-4
9	0.0029364997977715177
10-11	6.882421401026994E-5
12-13	5.7353511675224954E-5
14-15	2.2941404670089982E-4
16-17	1.261777256854949E-4
18-19	9.750096984788242E-4
20-21	2.638261537060348E-4
22-23	1.376484280205399E-4
24-25	0.0
26-27	0.0019385486946226036
28-29	0.011183934776668866
30-31	0.003349445081833138
32-33	0.013845137718399304
34-35	0.010988932836973102
36-37	0.012548948354539222
38-39	0.0123654171171785
40-41	0.013868079123069393
42-43	0.006343298391279881
44-45	0.0013764842802053991
46-47	0.005827116786202856
48-49	0.012835715912915347
50-51	0.003601800533204127
52-53	0.005551819930161776
54-55	0.004829165683053941
56-57	2.1794334436585483E-4
58-59	4.7029879573684465E-4
60-61	4.0147458172657465E-4
62-63	0.0011126581264993642
64-65	4.129452840616197E-4
66-67	0.001433837791880624
68-69	0.0018353123736071985
70-71	9.864804008138692E-4
72-73	0.0029479705001065628
74-75	0.007937726015851135
76-77	0.004060628626605927
78-79	0.0017091346479217039
80-81	5.391230097471146E-4
82-83	2.1794334436585483E-4
84-85	0.001640310433911434
86-87	0.004427691101327367
88-89	0.0017091346479217039
90-91	2.1794334436585483E-4
92-93	0.0022023748483286382
94-95	0.0034526814028485427
96-97	0.0018926658852824235
98-99	0.0021220799319833232
100	0.0023629646810192683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4358931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.05152760698108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.911529902945	49.263009808736335
2	13.89007521308369	17.793610719477474
3	4.295738158500121	8.254457737545977
4	1.819150157703844	4.6607738618964705
5	0.9385568929010487	3.0058001368186957
6	0.5588388759342919	2.1476690213855747
7	0.36538301046467714	1.638233798733009
8	0.24070860141473094	1.2334202903002756
9	0.17114297767535208	0.9865772243382386
>10	0.7707506971457546	8.232223233096008
>50	0.02757972389137997	1.2004077166922629
>100	0.010135789211865706	1.126716269108772
>500	2.0499957121096455E-4	0.09309549656066311
>1k	2.0499955700644E-4	0.36400468531023794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4782	0.10970579713237029	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.588280934017996E-5
2	0.0	0.0	0.0	0.0	4.588280934017996E-5
3	0.0	0.0	0.0	0.0	4.588280934017996E-5
4	0.0	0.0	0.0	0.0	4.588280934017996E-5
5	0.0	2.294140467008998E-5	0.0	0.0	4.588280934017996E-5
6	0.0	2.294140467008998E-5	0.0	0.0	6.882421401026994E-5
7	0.0	2.294140467008998E-5	0.0	0.0	6.882421401026994E-5
8	0.0	2.294140467008998E-5	0.0	0.0	6.882421401026994E-5
9	0.0	2.294140467008998E-5	0.0	6.882421401026994E-5	6.882421401026994E-5
10-11	0.0	2.294140467008998E-5	0.0	1.6058983269062987E-4	6.882421401026994E-5
12-13	0.0	3.441210700513497E-5	0.0	1.8353123736071985E-4	8.029491634531494E-5
14-15	0.0	4.588280934017996E-5	0.0	1.9500193969576484E-4	9.176561868035993E-5
16-17	2.294140467008998E-5	4.588280934017996E-5	0.0	2.1794334436585485E-4	1.376484280205399E-4
18-19	2.294140467008998E-5	9.176561868035993E-5	0.0	3.2117966538125974E-4	1.376484280205399E-4
20-21	2.294140467008998E-5	1.1470702335044992E-4	0.0	4.932402004069347E-4	1.376484280205399E-4
22-23	2.294140467008998E-5	1.1470702335044992E-4	0.0	6.767714377676544E-4	1.9500193969576487E-4
24-25	2.294140467008998E-5	1.1470702335044992E-4	0.0	9.864804008138694E-4	2.4088474903594483E-4
26-27	2.294140467008998E-5	1.1470702335044992E-4	0.0	0.0013535428755353089	2.523554513709898E-4
28-29	2.294140467008998E-5	1.1470702335044992E-4	0.0	0.0022482576576688183	2.523554513709898E-4
30-31	2.294140467008998E-5	1.1470702335044992E-4	0.0	0.006240062070264476	2.523554513709898E-4
32-33	2.294140467008998E-5	1.1470702335044992E-4	0.0	0.01725193631190767	2.523554513709898E-4
34-35	2.294140467008998E-5	1.1470702335044992E-4	0.0	0.0368668373048346	2.523554513709898E-4
36-37	2.294140467008998E-5	1.261777256854949E-4	0.0	0.06987951862509409	2.523554513709898E-4
38-39	2.294140467008998E-5	1.4911913035558488E-4	0.0	0.1196279546521842	2.523554513709898E-4
40-41	2.294140467008998E-5	1.6058983269062987E-4	0.0	0.17553615783319348	2.523554513709898E-4
42-43	2.294140467008998E-5	1.6058983269062987E-4	0.0	0.23494292522639154	2.6382615370603476E-4
44-45	2.294140467008998E-5	1.6058983269062987E-4	0.0	0.31351723622144967	2.867675583761248E-4
46-47	2.294140467008998E-5	1.6058983269062987E-4	0.0	0.40384901710992904	3.097089630462147E-4
48-49	2.294140467008998E-5	1.6058983269062987E-4	0.0	0.5037588344481709	3.900038793915297E-4
50-51	3.441210700513497E-5	1.6058983269062987E-4	0.0	0.6092090010142395	3.900038793915297E-4
52-53	4.588280934017996E-5	1.6058983269062987E-4	0.0	0.7144297535336072	4.358866887317097E-4
54-55	4.588280934017996E-5	1.9500193969576484E-4	0.0	0.8425230865090546	4.588280934017997E-4
56-57	4.588280934017996E-5	2.2941404670089984E-4	0.0	0.9915848633529643	4.588280934017997E-4
58-59	4.588280934017996E-5	2.2941404670089984E-4	0.0	1.1602271290827957	4.588280934017997E-4
60-61	4.588280934017996E-5	2.2941404670089984E-4	0.0	1.3324482539411613	4.588280934017997E-4
62-63	4.588280934017996E-5	2.2941404670089984E-4	0.0	1.5170921494283804	5.505937120821596E-4
64-65	4.588280934017996E-5	2.2941404670089984E-4	0.0	1.7092264135403843	5.505937120821596E-4
66-67	4.588280934017996E-5	2.523554513709898E-4	0.0	1.928420064460759	5.620644144172046E-4
68-69	4.588280934017996E-5	2.523554513709898E-4	0.0	2.1719545457360994	5.964765214223396E-4
70-71	4.588280934017996E-5	2.9823826071116976E-4	0.0	2.438384548872189	6.194179260924296E-4
72-73	9.176561868035993E-5	3.441210700513497E-4	0.0	2.709930485249709	6.538300330975645E-4
74-75	9.176561868035993E-5	3.441210700513497E-4	0.0	2.9836099722615472	6.653007354326095E-4
76-77	9.176561868035993E-5	3.441210700513497E-4	0.0	3.290118609356285	6.767714377676544E-4
78-79	9.176561868035993E-5	3.670624747214397E-4	0.0	3.6314867108472235	6.882421401026994E-4
80-81	9.176561868035993E-5	3.670624747214397E-4	0.0	3.987560252731691	6.882421401026994E-4
82-83	9.176561868035993E-5	3.670624747214397E-4	0.0	4.3628816331343625	6.882421401026994E-4
84-85	9.176561868035993E-5	3.670624747214397E-4	0.0	4.749616362360404	6.882421401026994E-4
86-87	9.176561868035993E-5	3.900038793915297E-4	0.0	5.149519457867077	7.111835447727894E-4
88	9.176561868035993E-5	3.900038793915297E-4	0.0	5.467005557096453	7.570663541129694E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6215	0.0	44.32425	1
GTATCAA	10575	0.0	40.947933	1
CAACGCA	12330	0.0	33.42751	5
ATCAACG	12470	0.0	32.977222	3
TATCAAC	13270	0.0	32.086678	2
AACGCAG	13680	0.0	30.780413	6
TCAACGC	13535	0.0	30.590399	4
ACGCAGA	15545	0.0	26.301533	7
GTACTAG	2305	0.0	26.194416	1
CGCAGAG	16220	0.0	24.975199	8
GTACATG	19560	0.0	24.742764	1
TACATGG	20015	0.0	23.950363	2
GTGGTAT	2885	0.0	23.707834	1
ACATGGG	20515	0.0	22.404734	3
TGGTATC	2990	0.0	21.533611	2
GCAGAGT	18400	0.0	21.530905	9
TAGGCAT	3210	0.0	20.789824	5
CATGGGG	16440	0.0	20.096525	4
ATGGGGG	11325	0.0	19.213667	5
CGTTAAC	770	0.0	18.990686	1
>>END_MODULE