FastQCFastQC Report
Thu 26 May 2016
ERR580285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6369109
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175310.27525043141827216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141850.22271561061366668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85140.13367646871799493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95650.050.6413081
GTATCAA177100.042.3009221
CAACGCA209500.034.620485
ATCAACG211250.034.3323363
TATCAAC225300.033.1152762
AACGCAG230450.032.0286836
TCAACGC237800.030.7175774
GTACTAG39950.030.1353231
ACGCAGA251450.028.7200857
GTGGTAT35550.028.4414331
CGCAGAG256400.028.0004778
GTACATG248600.026.9755951
GCATCGT40400.026.1674398
TACATGG255900.025.9220522
TAACGCC19300.025.572894
ACATGGG251450.025.5535353
TAGGCAT52750.025.1291545
GCAGAGT280550.024.939379
CTAACGC19450.024.891543
CATGGGG193400.024.2318464
Produced by FastQC (version 0.11.5)