FastQCFastQC Report
Thu 26 May 2016
ERR580285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6369109
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153280.24066160588553281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134580.21130114118003007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79790.12527654967123344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101950.048.3743061
GTATCAA183600.040.8683131
CAACGCA212800.033.992065
ATCAACG214650.033.743953
TATCAAC233100.031.9080982
AACGCAG238750.031.0039486
TCAACGC241200.030.3021774
GTGGTAT36950.028.2465231
ACGCAGA257400.027.9176457
TCTAACG18850.027.4358272
CGCAGAG261200.027.3853248
TAACGCC18650.026.462524
CTAACGC18950.026.291833
GTACATG253950.025.992341
CGCCTAC19950.025.2069727
TACATGG258700.025.0977022
ACATGGG252150.024.549973
GCAGAGT290100.024.364239
CTGTGCG49000.024.3607239
TAGGCAT54150.023.869585
Produced by FastQC (version 0.11.5)