FastQCFastQC Report
Thu 26 May 2016
ERR580284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6734795
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202170.30018731082386324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166390.24706022974715636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94600.14046455757005225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109700.052.5557751
GTATCAA198300.042.3778041
ATCAACG234750.034.6999933
CAACGCA235800.034.5268255
TATCAAC248150.033.703042
AACGCAG257600.032.1185766
TCAACGC257050.031.9112134
ACGCAGA278150.029.2059677
CGCAGAG286050.028.3174558
GTGGTAT41850.027.9795321
GTACATG259850.025.9696311
GCAGAGT314350.025.1883779
TACATGG264500.025.185562
ACATGGG262550.024.1510683
GTACTAG34600.023.7847481
GAGTACT175400.022.65322712-13
CATGGGG206250.022.4495544
TGGTATC49800.021.8067072
CTGTGCG45350.021.6562259
CAGAGTA313050.021.22595610-11
Produced by FastQC (version 0.11.5)