FastQCFastQC Report
Thu 26 May 2016
ERR580284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6734795
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179010.26579873626442974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154340.22916807415815926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91270.13552008635749122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109750.051.1048661
GTATCAA196400.041.8672751
CAACGCA237550.033.7749865
ATCAACG238850.033.5727233
TATCAAC250500.032.694392
AACGCAG261350.031.2897076
TCAACGC259400.030.9669514
GTGGTAT42750.029.033151
ACGCAGA286450.027.9737287
GTACTAG33450.027.4072111
CGCAGAG294800.027.0697968
GTACATG262650.024.8628671
GCAGAGT324000.024.149889
TACATGG267450.023.9423792
ACATGGG263750.023.4885253
TGGTATC51150.023.4383662
TAGGCAT46850.022.873995
CATGGGG208600.021.9017284
GAGTACT182800.021.4666412-13
CGTTAAC10550.021.3902131
Produced by FastQC (version 0.11.5)