FastQCFastQC Report
Thu 26 May 2016
ERR580283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442018
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218040.29298504787276786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163990.22035689781991927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109620.14729875686944052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101550.047.421441
GTATCAA181000.041.1558271
CAACGCA215400.033.5535935
ATCAACG221000.032.7496643
TATCAAC234400.031.5199092
GTACATG253250.030.9000051
AACGCAG239750.030.8965426
TCAACGC243200.029.9712814
TACATGG258300.029.6230342
ACATGGG256400.028.5215363
GTACTAG45050.027.6634121
ACGCAGA266250.027.2916157
CATGGGG205400.026.5350654
CGCAGAG275000.026.3374338
GCATCGT45150.024.359128
TAGTACT60250.024.273954
CTGTGCG57900.024.1909489
ATGGGGG152350.023.9513575
GTGGTAT39750.023.8984051
GCAGAGT303300.023.5086869
Produced by FastQC (version 0.11.5)