FastQCFastQC Report
Thu 26 May 2016
ERR580283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7442018
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191760.25767204540488886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151190.20315726191471184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109590.1472584452227877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102050.047.860441
GTATCAA182000.041.0934831
CAACGCA222900.032.640245
ATCAACG226750.032.0031283
TATCAAC241650.030.8471242
AACGCAG247950.030.3083446
TCAACGC245650.029.8852394
GTACATG250950.028.1918411
TACATGG256650.027.1580242
ACGCAGA272700.026.8165137
ACATGGG250300.026.4946773
GTACTAG42600.026.373181
CGCAGAG282600.025.810568
CATGGGG202800.024.7048234
CTAACGC17650.023.9657083
ATGGGGG150200.023.4684165
TCTAACG19050.023.1916242
GCAGAGT316750.022.7310759
TGGGGGG137750.022.313316
TAACGCC19200.022.2757664
Produced by FastQC (version 0.11.5)