FastQCFastQC Report
Thu 26 May 2016
ERR580282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7219129
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226880.3142761405150123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187230.2593526171924619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100000.13852086588285098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136500.050.845671
GTATCAA251150.041.6204261
CAACGCA287450.034.8479165
ATCAACG292800.034.256493
TATCAAC311950.033.1087572
GTGGTAT41350.032.523011
GTACTAG62650.032.2736171
AACGCAG326200.031.6747536
TAGGCAT76350.029.9830345
TCAACGC343700.029.321684
ACGCAGA339800.029.2739627
GCATCGT64500.028.7021568
CGCAGAG345750.028.6203028
CTAGGCA84700.027.5814174
GGGCACC64800.026.76652194
GCTATTG74800.026.3202748
GCAGAGT371250.026.2534589
GTACATG335150.025.7171881
AGGGCTA77000.025.578735
CTATTGA79150.025.1744049
Produced by FastQC (version 0.11.5)