FastQCFastQC Report
Thu 26 May 2016
ERR580282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7219129
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195690.2710714824461511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169610.2349452406239035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93960.13015420558352678No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136550.051.3406681
GTATCAA248550.040.8048671
CAACGCA284800.034.2591365
ATCAACG291100.033.5665973
TATCAAC313300.032.2807462
AACGCAG323400.030.8084496
GTACTAG62100.029.9832821
GGGCACC61650.029.95900794
GTGGTAT40150.029.745671
TCAACGC339250.029.09324
ACGCAGA340400.028.6483487
CGCAGAG345800.028.0378828
TATTCTC40350.027.6053855
TAGGCAT75600.026.7944285
TAGTACT79250.025.9756764
CTGTGCG78450.025.939229
GCAGAGT373650.025.6959889
CTAGGCA84250.025.1035214
GTACATG337050.024.1757411
AGGGCTA78400.023.9191575
Produced by FastQC (version 0.11.5)