FastQCFastQC Report
Thu 26 May 2016
ERR580281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623319
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140100.3866620631525957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120050.33132605768357687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63980.1765784354068742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70600.052.2144281
GTATCAA128550.040.4174921
GTGGTAT24100.035.1183931
CAACGCA148750.033.5877885
ATCAACG150450.033.1444133
TATCAAC161000.031.854442
AACGCAG166400.030.5234476
TCAACGC172100.029.2226774
TGGTATC29000.028.8531442
ACGCAGA177300.028.0128577
GTACATG164900.027.6871151
CGCAGAG180000.027.5661758
TACATGG170300.026.3884812
ACATGGG167400.025.8577843
GTACTAG25400.025.3609771
GCAGAGT193500.025.135799
CTGTGCG29950.024.4771549
CATGGGG128900.023.7745234
GCATCGT25500.023.7703028
ATGGGGG101000.023.5934645
Produced by FastQC (version 0.11.5)