FastQCFastQC Report
Thu 26 May 2016
ERR580281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623319
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127810.35274288573542656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113120.312199947065108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62780.1732665547802995No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69950.054.908661
GTATCAA128900.040.7386631
GTGGTAT22850.034.1529661
GTACTAG24100.033.5519641
ATCAACG153850.033.1455233
CAACGCA153100.033.124165
TATCAAC162000.032.299272
AACGCAG171100.030.2433536
TCAACGC173400.029.625774
ACGCAGA184050.027.5025227
TAGGCAT34000.027.0941665
CGCAGAG186350.027.0365948
TGGTATC29150.025.965692
GTACATG163250.025.5432991
CTAGGCA36800.025.160374
GCAGAGT197750.025.1445489
TACATGG166300.024.9055562
GCATCGT29300.024.862848
ACATGGG163300.024.0609473
GCTATTG29800.023.9725348
Produced by FastQC (version 0.11.5)