##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580281_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3623319
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51124121282172	34.0	31.0	34.0	31.0	34.0
2	32.632152730686975	34.0	31.0	34.0	31.0	34.0
3	32.678324486472206	34.0	31.0	34.0	31.0	34.0
4	36.204323715356004	37.0	35.0	37.0	35.0	37.0
5	36.14921429771985	37.0	35.0	37.0	35.0	37.0
6	36.18913267090201	37.0	36.0	37.0	35.0	37.0
7	36.141172499578424	37.0	36.0	37.0	35.0	37.0
8	36.1372250138616	37.0	36.0	37.0	35.0	37.0
9	37.84769488968539	39.0	38.0	39.0	35.0	39.0
10-11	37.80327994857754	39.0	38.0	39.0	35.0	39.0
12-13	37.76391645339535	39.0	38.0	39.0	35.0	39.0
14-15	39.12977466240207	40.0	38.5	41.0	36.0	41.0
16-17	39.11813823182557	40.0	38.0	41.0	36.0	41.0
18-19	39.122400760187006	40.0	38.5	41.0	36.0	41.0
20-21	39.101507761254254	40.0	39.0	41.0	35.0	41.0
22-23	39.022996457115696	40.0	38.5	41.0	35.0	41.0
24-25	38.9475109699146	40.0	38.0	41.0	35.0	41.0
26-27	38.8161150039508	40.0	38.0	41.0	35.0	41.0
28-29	38.7163375623289	40.0	38.0	41.0	35.0	41.0
30-31	38.5582028521364	40.0	38.0	41.0	34.0	41.0
32-33	38.43548484138438	40.0	38.0	41.0	34.0	41.0
34-35	38.33812038078899	40.0	38.0	41.0	34.0	41.0
36-37	38.21469500753315	40.0	38.0	41.0	34.0	41.0
38-39	38.096063029504165	40.0	37.0	41.0	33.5	41.0
40-41	37.979278390889675	40.0	37.0	41.0	33.0	41.0
42-43	37.972748604249304	40.0	37.0	41.0	33.0	41.0
44-45	37.90954288043642	40.0	37.0	41.0	33.0	41.0
46-47	37.69788735134831	40.0	37.0	41.0	33.0	41.0
48-49	37.47066198145954	40.0	36.5	41.0	32.5	41.0
50-51	37.269333862130274	40.0	36.0	41.0	32.0	41.0
52-53	37.10254934771131	39.5	36.0	41.0	32.0	41.0
54-55	36.88740875423886	39.0	35.0	41.0	31.0	41.0
56-57	36.64528185346087	39.0	35.0	41.0	31.0	41.0
58-59	36.39628197241259	38.5	35.0	40.5	31.0	41.0
60-61	36.14439040559222	38.0	35.0	40.0	30.5	41.0
62-63	35.8437047635055	37.5	35.0	40.0	30.0	41.0
64-65	35.54881311857996	37.0	35.0	40.0	29.5	41.0
66-67	35.235678393208	36.5	34.0	39.5	29.0	41.0
68-69	34.92363645044778	36.0	34.0	39.0	29.0	41.0
70-71	34.615647283609306	35.5	34.0	39.0	29.0	40.5
72-73	34.307534887212526	35.0	34.0	38.0	28.5	40.0
74-75	33.96370924006415	35.0	34.0	37.0	28.0	39.5
76-77	32.43856889222285	34.0	31.5	36.0	25.5	38.5
78-79	33.33072991917079	35.0	33.0	36.0	27.5	39.0
80-81	33.315460769532024	35.0	33.0	36.0	28.0	38.5
82-83	33.092051514095225	35.0	33.0	36.0	27.0	37.0
84-85	32.878377117775166	35.0	33.0	35.0	27.0	37.0
86-87	32.68254230444518	35.0	33.0	35.0	27.0	36.5
88-89	32.50224876694544	35.0	33.0	35.0	26.5	36.0
90-91	32.33459419388687	35.0	33.0	35.0	26.0	36.0
92-93	32.17178669612032	35.0	33.0	35.0	25.5	36.0
94-95	32.038927982879784	35.0	32.5	35.0	25.0	35.0
96-97	31.913275921882672	35.0	32.0	35.0	25.0	35.0
98-99	31.807779138408733	35.0	32.0	35.0	25.0	35.0
100	29.91944678346014	33.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0016523208340899487
1101	2	-0.061393225486156666
1101	3	-0.03107560041228652
1101	4	-0.04822167004688538
1101	5	-0.07807160766034116
1101	6	-0.012758504143818072
1101	7	-0.017772579443246173
1101	8	0.0028446399221309093
1101	9	-0.05035799068432567
1101	10-11	-0.060680987203021175
1101	12-13	-0.016033172001058915
1101	14-15	-0.08842769761287173
1101	16-17	-0.07566138293135083
1101	18-19	-0.0262655956422293
1101	20-21	-0.062494833654170634
1101	22-23	-0.17299789272883004
1101	24-25	-0.19300530297186214
1101	26-27	-0.17793646472265578
1101	28-29	-0.17778589463264183
1101	30-31	-0.14128675287020087
1101	32-33	-0.06521837612855563
1101	34-35	-0.14873098506029692
1101	36-37	-0.16646043106329245
1101	38-39	-0.16846836667608756
1101	40-41	-0.12780673918128826
1101	42-43	-0.18966545177886474
1101	44-45	-0.24117645067264704
1101	46-47	-0.2575188655234868
1101	48-49	-0.21802999197051065
1101	50-51	-0.2773840168509878
1101	52-53	-0.21889580040625845
1101	54-55	-0.23131757247303852
1101	56-57	-0.26624884603459265
1101	58-59	-0.24798620618058465
1101	60-61	-0.32872318084406515
1101	62-63	-0.34827252691969335
1101	64-65	-0.3293466544371171
1101	66-67	-0.34897024028377643
1101	68-69	-0.31929005600775184
1101	70-71	-0.2860634251538272
1101	72-73	-0.356699415136994
1101	74-75	-0.30523320299715095
1101	76-77	-0.34604363044433484
1101	78-79	-0.2543295472556011
1101	80-81	-0.2670001861733624
1101	82-83	-0.23537562005471813
1101	84-85	-0.29892737461778296
1101	86-87	-0.30932471029661457
1101	88-89	-0.3171568816862447
1101	90-91	-0.31862551183146337
1101	92-93	-0.2557647398985985
1101	94-95	-0.2859830640576746
1101	96-97	-0.39076603422308054
1101	98-99	-0.3720115020372212
1101	100	-0.502981658363634
1102	1	-0.037227121309555855
1102	2	-0.09505953038770087
1102	3	-0.06671315606136119
1102	4	0.0064767236667719885
1102	5	0.015180611534866273
1102	6	0.04377332503413811
1102	7	-0.009406289389652045
1102	8	0.009067902321611143
1102	9	-0.02916930105337201
1102	10-11	0.0058334674053028834
1102	12-13	0.020025581607363563
1102	14-15	-0.007030299860737443
1102	16-17	0.006257251909822514
1102	18-19	-0.061077982283009646
1102	20-21	-0.005950330392138881
1102	22-23	0.006205124769607551
1102	24-25	-0.015670913054698588
1102	26-27	0.01865765204133396
1102	28-29	0.0027734959612999432
1102	30-31	-0.10750076195780878
1102	32-33	-0.020979793263421698
1102	34-35	-0.0650126302794618
1102	36-37	-0.09743494918149764
1102	38-39	-0.09668309273130404
1102	40-41	-0.057062914003253695
1102	42-43	-0.045049476526550336
1102	44-45	-0.0284373144955552
1102	46-47	0.006520984421925391
1102	48-49	0.015542057121265884
1102	50-51	-0.06921124242425947
1102	52-53	-0.09059645740780553
1102	54-55	-0.08595714859260539
1102	56-57	-0.04429905509545051
1102	58-59	-0.1509920559471567
1102	60-61	-0.19060437586663426
1102	62-63	-0.08454116171984793
1102	64-65	-0.056973122518520825
1102	66-67	-0.02605213291418096
1102	68-69	-0.14663888371649136
1102	70-71	-0.1508029337603105
1102	72-73	-0.21846732271543345
1102	74-75	-0.22958532760841166
1102	76-77	-0.15837752406242345
1102	78-79	-0.0354105030702172
1102	80-81	-0.106405062045269
1102	82-83	-0.11641535433373207
1102	84-85	-0.09335180303299495
1102	86-87	-0.13714900073248515
1102	88-89	-0.16899339338454666
1102	90-91	-0.22874574746278142
1102	92-93	-0.19462639386247105
1102	94-95	-0.19824960865937413
1102	96-97	-0.23593507533223246
1102	98-99	-0.13728451165229671
1102	100	-0.06918483910755668
1103	1	-0.04222638146132596
1103	2	-0.013020931448856743
1103	3	-0.07891008252044429
1103	4	-0.06112567485071452
1103	5	-0.10473962578026175
1103	6	-0.02993105979967936
1103	7	-0.011120153675655331
1103	8	-0.06479314243802747
1103	9	-0.07781725778046678
1103	10-11	-0.024141307293298553
1103	12-13	0.027021375041734075
1103	14-15	-0.008475470061057422
1103	16-17	0.04227799584483449
1103	18-19	0.03719640980836658
1103	20-21	0.048975649554449774
1103	22-23	0.021200321627958374
1103	24-25	0.014503855537221
1103	26-27	0.007424289818693808
1103	28-29	-0.001306355975110307
1103	30-31	-0.049609164989547594
1103	32-33	-0.046204742354817085
1103	34-35	-0.022205011956586418
1103	36-37	-0.024723487640684993
1103	38-39	-0.08573545284713902
1103	40-41	-0.004193946995791009
1103	42-43	0.05445376823637815
1103	44-45	0.03631725553518805
1103	46-47	0.04180199756773817
1103	48-49	-0.015530863729068756
1103	50-51	0.003142625807598165
1103	52-53	0.0400845787555113
1103	54-55	0.0726247385136034
1103	56-57	0.043145368210474544
1103	58-59	-0.03196597805875001
1103	60-61	-0.002123930172672317
1103	62-63	0.041088355170998625
1103	64-65	0.09281537483798985
1103	66-67	0.0556550609941624
1103	68-69	0.015231100288687571
1103	70-71	0.013086013846695721
1103	72-73	0.01107916206600379
1103	74-75	0.06215679183362255
1103	76-77	0.005352532330455517
1103	78-79	0.10644576438374287
1103	80-81	0.028788343675756778
1103	82-83	0.04145933181795414
1103	84-85	0.015817265204496778
1103	86-87	-0.005889708591077181
1103	88-89	0.05391653537864016
1103	90-91	-0.014789861206814692
1103	92-93	-0.11351918163406793
1103	94-95	-0.020148411514483655
1103	96-97	-0.10729191000316263
1103	98-99	-0.07928534310078206
1103	100	0.03508608156896997
1104	1	-0.015287768276152747
1104	2	0.0034408176328000195
1104	3	-0.017441889899494356
1104	4	0.022176783896583174
1104	5	0.03006510856907596
1104	6	-0.04317039876179507
1104	7	-1.6738015074224677E-4
1104	8	0.036228578426623415
1104	9	0.05950483848449295
1104	10-11	0.015001639817235457
1104	12-13	-0.026826766300651173
1104	14-15	-0.018765504302272973
1104	16-17	-0.01636431504955027
1104	18-19	-0.01868674161921291
1104	20-21	0.025059468084073444
1104	22-23	-0.01989203817976204
1104	24-25	-0.004889151985032925
1104	26-27	-0.013322151051710307
1104	28-29	-0.02304677390888088
1104	30-31	0.040554346366008076
1104	32-33	0.005693458841690813
1104	34-35	0.08224855383393503
1104	36-37	0.08001851723550857
1104	38-39	0.120151959612393
1104	40-41	0.07152839587404713
1104	42-43	0.07112468840001185
1104	44-45	0.08921580085429781
1104	46-47	0.10928167365094055
1104	48-49	0.16582340298802478
1104	50-51	0.1421050875101102
1104	52-53	0.09899999504852985
1104	54-55	0.11603512230561108
1104	56-57	0.1153985303219045
1104	58-59	0.1115963260270263
1104	60-61	0.16262979310649683
1104	62-63	0.20901479536777146
1104	64-65	0.13844183431677948
1104	66-67	0.1497662620980691
1104	68-69	0.12945327961175224
1104	70-71	0.1751263903455964
1104	72-73	0.1956232501083477
1104	74-75	0.12326918263573106
1104	76-77	0.10926727160541105
1104	78-79	0.15526750804925626
1104	80-81	0.1728086616022395
1104	82-83	0.324976040224648
1104	84-85	0.21895644392015612
1104	86-87	0.2333804591443922
1104	88-89	0.24627451209449447
1104	90-91	0.1715157170981101
1104	92-93	0.23708116423664194
1104	94-95	0.16162163547290476
1104	96-97	0.05492764835198827
1104	98-99	0.13559684030775188
1104	100	0.24522360025289558
1105	1	0.040213250023946046
1105	2	0.033872606178022124
1105	3	0.03409633978630211
1105	4	0.06310135433719921
1105	5	0.0682890383074124
1105	6	0.0631294566040097
1105	7	0.09961451734107385
1105	8	0.08240424932208157
1105	9	0.04556731375958378
1105	10-11	0.01044414510758429
1105	12-13	0.006808010374520279
1105	14-15	0.0836113316205882
1105	16-17	0.0586463598332827
1105	18-19	0.03222538525700003
1105	20-21	0.03345468782967487
1105	22-23	0.06391599603010434
1105	24-25	0.020090597134291954
1105	26-27	-0.03296576694849307
1105	28-29	0.04422827386645878
1105	30-31	-0.05659650647339731
1105	32-33	-0.04189689566907617
1105	34-35	0.10830965811916826
1105	36-37	0.12536950110386869
1105	38-39	0.0561767065025478
1105	40-41	0.15013477945397824
1105	42-43	0.07170317285968508
1105	44-45	0.1523276443228312
1105	46-47	0.09349409754007354
1105	48-49	0.09786783143177047
1105	50-51	0.11328735171555593
1105	52-53	0.09239752768541365
1105	54-55	0.05971621959984219
1105	56-57	0.08420013867091569
1105	58-59	0.1381226249614187
1105	60-61	0.0942108583760799
1105	62-63	0.041187322405576765
1105	64-65	0.10590463444320619
1105	66-67	0.11774553817355837
1105	68-69	0.0434644819593899
1105	70-71	0.1350147331642333
1105	72-73	0.17167200501931035
1105	74-75	0.1029293446009163
1105	76-77	0.055578628634187055
1105	78-79	0.2210870564807763
1105	80-81	0.2110409637977071
1105	82-83	0.19203790431036794
1105	84-85	0.2042240590466804
1105	86-87	0.20416790966840637
1105	88-89	0.17558899623600865
1105	90-91	0.24264732793792376
1105	92-93	0.20455416829519635
1105	94-95	0.2270416607158623
1105	96-97	0.13489821916518352
1105	98-99	0.24810984378022383
1105	100	0.18648924366576125
1106	1	-0.013359629092811076
1106	2	-0.05371206475647483
1106	3	-0.04526284648007817
1106	4	0.012302215440421094
1106	5	-0.03412385086646452
1106	6	-0.025612526343529396
1106	7	-0.05134648753721649
1106	8	-0.0017276030168673628
1106	9	0.025564591197579034
1106	10-11	-7.575238377981464E-4
1106	12-13	-0.012337030768890145
1106	14-15	0.03222297377180183
1106	16-17	-0.07080458272363188
1106	18-19	0.03358276094526502
1106	20-21	0.02510865912523741
1106	22-23	-0.036148237092618274
1106	24-25	-0.010901456852771219
1106	26-27	-0.005714148660615592
1106	28-29	-0.027447894492013347
1106	30-31	0.02008675839914531
1106	32-33	-0.03487830190954355
1106	34-35	0.01672732144685085
1106	36-37	-0.026195302566677015
1106	38-39	0.0782705575293079
1106	40-41	0.01791191915712176
1106	42-43	0.0177133177505695
1106	44-45	0.038054836171220074
1106	46-47	0.035067954101222654
1106	48-49	0.030389132132050634
1106	50-51	0.08522647790704951
1106	52-53	0.054903050915712015
1106	54-55	0.01574409089854356
1106	56-57	0.043322357901139696
1106	58-59	-0.0027755006364742485
1106	60-61	-0.09863560995516707
1106	62-63	-0.06559237769603499
1106	64-65	0.03795153243341787
1106	66-67	0.039448113895751646
1106	68-69	-0.0391080713425751
1106	70-71	0.013256292850080342
1106	72-73	0.03522102824440054
1106	74-75	0.02180205515687561
1106	76-77	-0.03051365431587527
1106	78-79	0.06900972679339645
1106	80-81	0.1390303145633638
1106	82-83	0.04874518872586009
1106	84-85	0.009202776943133983
1106	86-87	0.11798736661398124
1106	88-89	-0.0031172958576988208
1106	90-91	0.05362308374643021
1106	92-93	0.10744350581997963
1106	94-95	0.08891705217713763
1106	96-97	0.0206065539242708
1106	98-99	0.0991784493672796
1106	100	0.09516997640687563
1107	1	-0.01472217420683819
1107	2	-0.021576700155485184
1107	3	-0.001977782354074975
1107	4	-0.005272324788030858
1107	5	-0.013189303554440812
1107	6	-0.046155096391423456
1107	7	-0.06666782816196815
1107	8	-0.07461267994091969
1107	9	-0.09599345146082783
1107	10-11	-0.12282138681541
1107	12-13	-0.0145233754804579
1107	14-15	-0.027454783415841177
1107	16-17	-0.04615663121035851
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2316	34-35	-0.15882811711820466
2316	36-37	-0.12236656364799359
2316	38-39	-0.08456110619071211
2316	40-41	-0.11792793184501704
2316	42-43	-0.17756502299558719
2316	44-45	-0.11818847633974627
2316	46-47	-0.13942226268411417
2316	48-49	-0.10953390051283662
2316	50-51	-0.07070957182956761
2316	52-53	-0.13711707718423582
2316	54-55	-0.1264858705166816
2316	56-57	-0.27991253722056086
2316	58-59	-0.22549868626774128
2316	60-61	-0.05519155440730117
2316	62-63	-0.09326516811827901
2316	64-65	-0.0180165579805589
2316	66-67	-0.09997192543863065
2316	68-69	-0.12520747275020483
2316	70-71	-0.059256710818736735
2316	72-73	-0.10839926846133352
2316	74-75	-0.055327630094900826
2316	76-77	-0.04528914818335039
2316	78-79	-0.1366064217079952
2316	80-81	-0.18178106416545603
2316	82-83	-0.1786400374886412
2316	84-85	-0.1368625128227805
2316	86-87	-0.09107324186602028
2316	88-89	-0.1086475376898548
2316	90-91	-0.15243790371307853
2316	92-93	-0.13341400590589814
2316	94-95	-0.07749542744007698
2316	96-97	-0.09635286598257764
2316	98-99	-0.018972332875001996
2316	100	-0.2100647171967367
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	26.0
14	132.0
15	479.0
16	1315.0
17	3044.0
18	5686.0
19	9044.0
20	13152.0
21	20159.0
22	26440.0
23	26769.0
24	24506.0
25	23279.0
26	25801.0
27	31471.0
28	38266.0
29	46868.0
30	57709.0
31	73395.0
32	93021.0
33	123691.0
34	187473.0
35	281132.0
36	485195.0
37	881844.0
38	913029.0
39	230307.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.802318552894434	21.216205250609832	13.877313543402622	23.104162653093105
2	15.599802770380439	21.03281068220356	40.23056319216029	23.13682335525571
3	18.133037615682603	27.35877697420787	30.182188715405616	24.325996694703907
4	12.579168686017846	17.726968632806333	38.862565258622304	30.83129742255351
5	12.81239744537021	38.52115430316122	34.99461368248852	13.671834568980055
6	29.57330822191152	38.33124776792984	18.0439862027015	14.051457807457144
7	26.10965156679989	33.08438742055879	23.38228625633646	17.42367475630486
8	25.699319807501436	35.34279560110535	20.255130148502992	18.702754442890214
9	26.305230526695787	15.54306774988767	21.409346479476536	36.74235524394
10-11	24.983878693255797	26.362397039944547	27.989596806566812	20.664127460232844
12-13	25.559104329112753	24.355745804776976	28.16064283629574	21.924507029814528
14-15	23.131669356672724	25.599538319890662	26.011567394035474	25.25722492940114
16-17	21.732796458714	28.15909126348747	27.1566533036791	22.95145897411943
18-19	21.288879762734712	27.666129585133493	29.578095684414645	21.46689496771715
20-21	22.571344275995077	26.91783840801963	29.206932554230097	21.303884761755203
22-23	22.92715332681617	26.47666986636874	28.86804237296088	21.72813443385421
24-25	22.40010145514722	26.64039394183606	29.256576093016374	21.70292851000034
26-27	22.107589072680348	26.946781742022146	28.81401471441792	22.13161447087959
28-29	21.58347223719089	27.18460843601685	29.25709216946551	21.97482715732675
30-31	22.420580214376773	26.78544962570865	29.170823849436704	21.623146310477868
32-33	22.492363256194952	27.109281334661045	28.587875820529067	21.810479588614932
34-35	22.448514347867725	27.10080421362789	29.02826463565858	21.422416802845813
36-37	22.362837289284975	26.731843486759317	28.774847547154504	22.130471676801207
38-39	22.33094167705547	26.894734648693685	29.091397847978534	21.682925826272303
40-41	22.09963072591152	26.334697646921313	29.547971476512615	22.017700150654555
42-43	21.991683551001177	26.57544572021105	28.925383523483923	22.507487205303846
44-45	22.08172068058615	27.11950388209665	28.79618433686956	22.002591100447646
46-47	22.092402584212106	26.87436244767103	28.26584110539982	22.767393862717043
48-49	22.552728899711134	26.843356846604166	28.351531242362142	22.252383011322554
50-51	22.40142619794127	26.868451925782615	28.1082190342168	22.621902842059317
52-53	21.916671312721046	27.32070416152151	27.94797670311933	22.814647822638115
54-55	21.911070538709037	27.24734882122682	27.928095767429056	22.913484872635088
56-57	22.495868646772234	26.92895728439511	27.615269067532015	22.959905001300644
58-59	21.797758174036836	27.157106116886066	28.21907815312667	22.826057555950428
60-61	22.109701566792626	27.458333661096894	27.792252276645694	22.639712495464785
62-63	22.24721085834976	26.996586583278194	27.690378884426142	23.06582367394591
64-65	21.602246182842936	26.99464163511204	27.897527981721197	23.505584200323828
66-67	22.103348877672236	26.807250197616238	27.70749429231306	23.381906632398465
68-69	21.72768919296922	26.897456515766212	27.87466240491759	23.50019188634698
70-71	21.731823654130963	26.529670749431855	27.94253860815776	23.795966988279417
72-73	21.994511311897476	26.797375608872876	27.830900049902183	23.377213029327468
74-75	22.1751533476058	27.03720432965564	27.536575096268773	23.251067226469782
76-77	21.88841024033138	26.942238882921117	27.384697463322112	23.784653413425396
78-79	21.840223943983645	26.877335193553257	27.401232740698727	23.881208121764367
80-81	21.63088167167669	27.20562127258323	27.522106271561352	23.641390784178732
82-83	22.15513959080009	26.551680881532846	28.148471072150468	23.144708455516597
84-85	22.19967333087145	26.813364178671122	27.313331335120683	23.673631155336746
86-87	21.350447864014054	27.257950020948346	27.74713112559678	23.64447098944082
88-89	21.831730995464216	26.42670030451526	27.67294009231089	24.06862860770963
90-91	21.84259177610884	26.36852911900759	27.66152199103542	24.12735711384815
92-93	22.003123938729512	26.478975511483217	27.528664739055625	23.98923581073165
94-95	21.265524463924898	26.360642553211456	27.89503186794777	24.47880111491588
96-97	21.47918990487417	26.298518720812663	27.694998431658174	24.527292942654995
98-99	21.095064850187978	26.61813597315577	27.546992029860284	24.739807146795965
100	21.193088765601033	26.41620194528882	27.1909338346786	25.199775454431546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	889.0
1	610.5
2	463.5
3	586.5
4	467.0
5	449.5
6	752.0
7	1441.5
8	2358.0
9	2881.0
10	2590.0
11	2177.5
12	2275.0
13	2570.0
14	2868.0
15	3215.0
16	3599.0
17	4103.5
18	4767.0
19	5311.5
20	5836.0
21	6459.0
22	7227.0
23	8304.0
24	9559.0
25	10983.5
26	13031.5
27	15718.0
28	18987.0
29	24168.5
30	31884.0
31	39813.0
32	49999.5
33	62314.0
34	73759.5
35	84256.0
36	96656.5
37	112955.5
38	119695.0
39	118136.0
40	114014.0
41	108406.0
42	107625.0
43	112029.0
44	119500.0
45	133454.5
46	146109.0
47	153395.5
48	158248.5
49	156960.0
50	152733.5
51	150870.5
52	147925.5
53	140598.5
54	133951.5
55	119930.0
56	105287.0
57	88774.5
58	71987.0
59	63896.5
60	51488.0
61	37592.0
62	28267.5
63	21890.5
64	15533.5
65	9077.0
66	5817.0
67	4192.5
68	3065.5
69	2158.5
70	1552.0
71	1115.0
72	762.0
73	501.5
74	371.5
75	254.0
76	146.0
77	78.5
78	37.5
79	20.5
80	12.0
81	6.0
82	3.5
83	1.5
84	1.0
85	1.0
86	1.0
87	0.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005437004028626792
2	0.03160086097856689
3	0.0011867572245225992
4	0.0011867572245225992
5	0.006899751305363949
6	2.75990052214558E-5
7	0.0011591582193011434
8	0.006264974185270466
9	0.0014075492662942457
10-11	2.345915443823743E-4
12-13	4.139850783218369E-5
14-15	8.555691618651298E-4
16-17	6.89975130536395E-5
18-19	9.107671723080414E-4
20-21	2.207920417716464E-4
22-23	0.001793935339394627
24-25	0.0011867572245225992
26-27	0.001931930365501906
28-29	2.621905496038301E-4
30-31	0.0019871283759448177
32-33	0.0012971532454084224
34-35	7.727721462007623E-4
36-37	0.004126051280607642
38-39	7.727721462007623E-4
40-41	0.003574071176178526
42-43	0.0038086627205609004
44-45	0.0040846527727754585
46-47	0.002401113454266654
48-49	0.009093872220469685
50-51	0.0071619418549677795
52-53	0.004774627903311853
54-55	0.0045952343693723904
56-57	0.0030772890821923215
58-59	0.0055474000495126154
60-61	0.007741520964618351
62-63	0.0032152841082996003
64-65	0.0029116950508635865
66-67	0.0037258657048965327
68-69	0.0022217199203271917
70-71	0.0033394796317961517
72-73	0.006375370206156289
74-75	0.0071619418549677795
76-77	0.004277845809325649
78-79	0.0025253089777632055
80-81	0.002276917930770103
82-83	0.004843625416365493
84-85	0.0022355194229379195
86-87	0.0033394796317961517
88-89	5.381806018183881E-4
90-91	0.001614541805455164
92-93	2.345915443823743E-4
94-95	0.0023735144490451983
96-97	0.0018077348420053548
98-99	0.001241955234965511
100	2.75990052214558E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3623319.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.11447191417312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30193516913982	48.15756344229734
2	14.869468471847307	18.769573004901797
3	4.391832075514776	8.31564486545526
4	1.7520022193418596	4.423067794648832
5	0.8673055735501521	2.7369766631418444
6	0.4726204592462688	1.789751442069734
7	0.2893770792815256	1.2784717080043508
8	0.20191292520279078	1.0194902117456062
9	0.14560080748021445	0.8270566266951831
>10	0.6510333905206384	6.93120777116473
>50	0.03380172668717983	1.4822638138536062
>100	0.021266417194025583	2.4292950370545805
>500	0.0014851906896366624	0.6345350200323809
>1k	2.0485388787449283E-4	0.2234173527837593
>5k	5.1213471968623206E-5	0.2029244995336286
>10k+	1.0242694393724641E-4	0.7787607466173485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12781	0.35274288573542656	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11312	0.312199947065108	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6278	0.1732665547802995	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.519801044291159E-5
2	0.0	0.0	0.0	0.0	5.519801044291159E-5
3	0.0	0.0	0.0	0.0	5.519801044291159E-5
4	0.0	0.0	0.0	0.0	5.519801044291159E-5
5	0.0	0.0	0.0	0.0	5.519801044291159E-5
6	0.0	0.0	0.0	0.0	5.519801044291159E-5
7	0.0	0.0	0.0	0.0	5.519801044291159E-5
8	0.0	0.0	0.0	0.0	5.519801044291159E-5
9	0.0	0.0	0.0	0.0	5.519801044291159E-5
10-11	0.0	0.0	0.0	0.0	5.519801044291159E-5
12-13	0.0	0.0	0.0	0.0	1.2419552349655108E-4
14-15	0.0	0.0	0.0	0.0	1.517945287180069E-4
16-17	0.0	0.0	0.0	1.3799502610727898E-5	1.655940313287348E-4
18-19	0.0	0.0	0.0	2.7599005221455797E-5	1.931930365501906E-4
20-21	0.0	0.0	0.0	5.519801044291159E-5	2.4839104699310216E-4
22-23	0.0	0.0	0.0	1.37995026107279E-4	2.6219054960383004E-4
24-25	0.0	0.0	0.0	3.449875652681975E-4	2.75990052214558E-4
26-27	0.0	0.0	0.0	0.0013523512558513342	3.311880626574696E-4
28-29	0.0	0.0	0.0	0.004250246804104193	3.311880626574696E-4
30-31	0.0	0.0	0.0	0.016518004625041292	3.311880626574696E-4
32-33	0.0	0.0	0.0	0.05061657557614993	3.5878706787892536E-4
34-35	0.0	0.0	0.0	0.1053592024329075	3.5878706787892536E-4
36-37	0.0	0.0	0.0	0.1956217490096787	3.5878706787892536E-4
38-39	0.0	0.0	0.0	0.3234741406980727	4.13985078321837E-4
40-41	0.0	0.0	0.0	0.4567083384046505	4.2778458093256487E-4
42-43	0.0	0.0	0.0	0.5926058401150989	4.4158408354329275E-4
44-45	0.0	0.0	0.0	0.7613875565469119	4.8298259137547644E-4
46-47	0.0	0.0	0.0	0.9696220509427957	5.243810992076602E-4
48-49	0.0	0.0	0.0	1.1923184240747227	5.243810992076602E-4
50-51	0.0	0.0	0.0	1.4114131270252495	5.243810992076602E-4
52-53	0.0	0.0	0.0	1.6274995384066377	5.243810992076602E-4
54-55	0.0	0.0	0.0	1.8755041993266395	5.243810992076602E-4
56-57	0.0	0.0	0.0	2.1719865129181284	5.243810992076602E-4
58-59	0.0	0.0	0.0	2.503105578062544	5.243810992076602E-4
60-61	0.0	0.0	0.0	2.8425733422864505	5.243810992076602E-4
62-63	0.0	0.0	0.0	3.1839868363784696	5.51980104429116E-4
64-65	0.0	0.0	0.0	3.541421553001544	5.51980104429116E-4
66-67	0.0	0.0	0.0	3.941717524733538	5.657796070398438E-4
68-69	0.0	0.0	0.0	4.383329207282053	5.795791096505717E-4
70-71	0.0	0.0	0.0	4.8650974424277855	5.795791096505717E-4
72-73	2.7599005221455797E-5	0.0	0.0	5.3377580058504375	6.623761253149392E-4
74-75	2.7599005221455797E-5	0.0	0.0	5.8093284085668415	6.623761253149392E-4
76-77	4.139850783218369E-5	0.0	0.0	6.336248616254876	6.623761253149392E-4
78-79	5.519801044291159E-5	0.0	0.0	6.909190165149687	6.89975130536395E-4
80-81	5.519801044291159E-5	0.0	0.0	7.5180104208323915	7.037746331471228E-4
82-83	5.519801044291159E-5	0.0	0.0	8.136338533813888	7.175741357578507E-4
84-85	5.519801044291159E-5	0.0	0.0	8.740259966069782	7.175741357578507E-4
86-87	5.519801044291159E-5	0.0	0.0	9.363804291038134	7.313736383685787E-4
88	5.519801044291159E-5	0.0	0.0	9.853838428247693	7.451731409793066E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6995	0.0	54.90866	1
GTATCAA	12890	0.0	40.738663	1
GTGGTAT	2285	0.0	34.152966	1
GTACTAG	2410	0.0	33.551964	1
ATCAACG	15385	0.0	33.145523	3
CAACGCA	15310	0.0	33.12416	5
TATCAAC	16200	0.0	32.29927	2
AACGCAG	17110	0.0	30.243353	6
TCAACGC	17340	0.0	29.62577	4
ACGCAGA	18405	0.0	27.502522	7
TAGGCAT	3400	0.0	27.094166	5
CGCAGAG	18635	0.0	27.036594	8
TGGTATC	2915	0.0	25.96569	2
GTACATG	16325	0.0	25.543299	1
CTAGGCA	3680	0.0	25.16037	4
GCAGAGT	19775	0.0	25.144548	9
TACATGG	16630	0.0	24.905556	2
GCATCGT	2930	0.0	24.86284	8
ACATGGG	16330	0.0	24.060947	3
GCTATTG	2980	0.0	23.972534	8
>>END_MODULE