FastQCFastQC Report
Thu 26 May 2016
ERR580280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8448021
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121370.14366678302527894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94580.11195521412648005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.042.0575071
GTATCAA144300.040.37461
CAACGCA157500.035.303645
ATCAACG163000.034.0833783
GTACATG270600.032.5297931
TATCAAC176050.032.418142
AACGCAG179100.031.6871646
TACATGG275150.031.5233422
TCAACGC182050.030.8532034
ACATGGG275150.030.2525373
GTACTAG38650.029.4421231
CATGGGG221650.028.7551064
ATGGGGG163100.027.6651255
ACGCAGA201900.027.3181657
TAACGCC17700.027.0863174
CTAACGC17800.026.9335083
TCTAACG18250.026.5325982
GAGTACA187850.025.9079111
CGCAGAG213850.025.7913158
AGTACAT187450.024.9040052
Produced by FastQC (version 0.11.5)