FastQCFastQC Report
Thu 26 May 2016
ERR580280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8448021
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105870.12531929075460393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88600.10487663323753575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83200.042.5546381
GTATCAA140000.039.025941
CAACGCA157000.033.139885
GTACATG267550.032.4064331
ATCAACG165700.031.513723
TACATGG271550.031.1322122
ACATGGG268350.030.4408173
TATCAAC178500.030.1340772
AACGCAG177850.029.9927776
TCAACGC180100.029.098414
GTACTAG39800.027.7625921
CTAACGC15800.027.6652093
CATGGGG216700.027.3938164
TAACGCC16550.027.2634834
ATGGGGG156500.026.0380065
TCTAACG17200.025.6962362
ACGCAGA202000.025.662497
CTGTGCG55000.024.5234229
TGGGGGG136300.024.378016
GAGTACA186300.024.3298511
Produced by FastQC (version 0.11.5)