FastQCFastQC Report
Thu 26 May 2016
ERR580279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505131
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168160.16007415804714856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137770.1311454374057782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144450.039.980551
GTATCAA235200.036.7315941
GTACTAG87800.035.0844541
CAACGCA248800.032.507375
ATCAACG264800.030.7070943
TAGTACT122050.030.13094
GCATCGT94950.030.0471328
TAGGCAT113100.029.5188585
GGGCACC107300.029.43333894
GTACATG395750.029.138521
TATCAAC295350.029.092422
CTGTGCG125350.028.7970689
TACATGG399750.028.565282
AACGCAG297000.028.1843816
ACATGGG393300.028.0282143
CTAGGCA125000.027.7268524
CTAGTAC137300.027.7102053
GTACTGT142150.026.8809576
CTAACGC39800.026.8228263
TCAACGC313900.026.3672284
Produced by FastQC (version 0.11.5)