FastQCFastQC Report
Thu 26 May 2016
ERR580279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505131
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149060.1418925665943623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127500.1213692623157198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145950.038.2965281
GTATCAA236950.037.3572771
CAACGCA258300.032.0251665
GTACTAG82550.031.4912381
ATCAACG270550.030.5754153
GGGCACC103600.029.4471994
TATCAAC304000.028.8669362
TAGTACT121800.028.1694684
AACGCAG304500.028.1062556
CTGTGCG122900.028.0691139
GTACATG389550.027.8880651
TACATGG394650.027.1432722
ACATGGG387150.026.599313
CTAGTAC135350.026.1830543
TCAACGC320000.026.1440664
ACGCAGA326500.025.3919327
CTAACGC36150.024.8331763
GTACTGT142400.024.5881946
CATGGGG326000.024.4806614
TACGGGC71450.024.4047744
Produced by FastQC (version 0.11.5)