FastQCFastQC Report
Thu 26 May 2016
ERR580278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581071
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232880.24306259707291594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164490.17168226808881804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111120.11597868338518731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142800.041.234711
GTATCAA252800.040.4454151
CAACGCA286600.034.325045
GTACTAG80150.033.153681
ATCAACG299550.032.924083
TATCAAC322000.031.6080192
AACGCAG334800.030.267126
GCATCGT79450.029.8737248
GTACATG382000.029.0682871
GGGCACC95300.029.04726694
ACGCAGA348300.028.2300387
TACATGG390150.028.0879612
TCAACGC355600.028.0641774
CGCAGAG357050.027.524348
TAGGCAT102400.027.5075825
ACATGGG386050.027.3012713
CTAACGC41400.026.696363
TAGTACT114100.026.626594
TAACGCC42700.025.9927854
CTGTGCG117400.025.7819949
Produced by FastQC (version 0.11.5)