FastQCFastQC Report
Thu 26 May 2016
ERR580278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9581071
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203770.21267977243880146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148390.1548783011836568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109960.11476796278829371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146250.040.9189871
GTATCAA256850.040.0460851
CAACGCA294150.033.491745
ATCAACG306450.032.209333
GTACTAG79950.031.575351
TATCAAC334050.030.6467442
AACGCAG347550.029.5067256
GGGCACC95150.029.24616894
TCAACGC356000.028.095364
TAGTACT111250.027.7996234
CTGTGCG112850.027.570069
GTACATG392300.027.4176391
ACGCAGA361700.027.2867647
TAGGCAT102950.027.1191045
TACATGG397750.026.71952
CGCAGAG371500.026.5416538
ACATGGG389250.025.9978543
CTAGGCA113800.025.7724294
CTAACGC35200.025.2372043
AGGGCTA87250.025.0497155
Produced by FastQC (version 0.11.5)