FastQCFastQC Report
Thu 26 May 2016
ERR580277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5936693
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174750.2943557970742297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145460.24501856504959918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84030.14154344851586564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90150.052.7284361
GTATCAA164750.042.0089531
ATCAACG191950.035.0122953
CAACGCA194700.034.351125
TATCAAC207800.033.1832962
AACGCAG209700.032.3291366
TCAACGC210150.032.049044
ACGCAGA230650.028.7820827
CGCAGAG233750.028.259688
GTACATG192300.027.4818821
GTGGTAT36600.026.977251
TACATGG195700.026.4442582
ACATGGG190650.026.2287433
GCAGAGT257650.025.2780469
CATGGGG138100.024.4019074
CTGTGCG32450.024.327929
ATGGGGG107050.023.7965815
GTACTAG31400.022.760061
TGGTATC42200.021.9426292
TAGTACT42300.021.3333574
Produced by FastQC (version 0.11.5)