FastQCFastQC Report
Thu 26 May 2016
ERR580277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5936693
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151360.2549567579121912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135850.2288311017598518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80220.13512573414188675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89150.055.6347161
GTATCAA161450.040.3880771
CAACGCA194200.032.50285
ATCAACG194850.032.4919553
TATCAAC208550.031.1077772
TCAACGC208100.030.467554
AACGCAG212200.030.1209896
ACGCAGA232300.027.0089557
CGCAGAG234050.026.706388
GTGGTAT37950.025.7671451
GTACATG189200.025.6432631
TACATGG193950.024.5053832
GCAGAGT258950.023.7923249
ACATGGG192400.023.76933
TGGTATC38100.023.5672262
CTGTGCG32300.022.260719
GGCGTCG21050.022.10328
CATGGGG141000.021.899994
ACGGGCG22250.020.9121745
GAGTACT169750.020.77748112-13
Produced by FastQC (version 0.11.5)