FastQCFastQC Report
Thu 26 May 2016
ERR580276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4036657
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.11957914680390234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.10203988102035917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.047.2910651
GTATCAA105750.040.367021
CAACGCA119250.034.138395
ATCAACG124550.032.8005453
TATCAAC130000.032.403852
AACGCAG134050.031.0587236
TCAACGC136600.030.0107084
GTACTAG24000.029.7751241
ACGCAGA149150.027.0314877
CGCAGAG152550.026.3058058
GTACATG190300.025.198961
GCATCGT24750.024.8715028
TACATGG193050.024.5727272
TAGGCAT32300.024.1595635
ACATGGG194400.023.4332523
GCAGAGT170050.022.497239
CATGGGG150200.022.3480034
GTGATCG15150.022.021748
GTATAAG26600.021.5625151
GTGGTAT25200.021.0813851
Produced by FastQC (version 0.11.5)