FastQCFastQC Report
Thu 26 May 2016
ERR580276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4036657
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42510.1053099136240706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.046.9643861
GTATCAA106400.038.110811
CAACGCA121350.031.6790055
ATCAACG125050.030.817613
TATCAAC132050.030.4292412
AACGCAG136900.029.0758636
GTACTAG23900.028.2117161
TCAACGC138150.028.0650924
ACGCAGA150550.025.4407277
CGCAGAG156800.024.1868938
GTGGTAT27550.024.1317521
GTACATG189900.022.4457871
TAGGCAT32350.022.371985
CGTTAAC7100.021.9152761
TGGTATC29650.021.8734762
TACATGG192000.021.7602522
GCAGAGT174200.021.0153489
ACATGGG192850.020.9578933
TACCCCG4750.020.7770425
CTAGGCA38050.019.8854144
Produced by FastQC (version 0.11.5)