FastQCFastQC Report
Thu 26 May 2016
ERR580275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639250
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45760.12574019372123377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46250.042.395731
GTATCAA79250.040.7619741
CAACGCA91900.033.86735
ATCAACG94450.032.905873
TATCAAC98050.032.7069852
AACGCAG101700.031.053696
TCAACGC100250.031.0485634
ACGCAGA114550.026.954747
GTACATG135300.025.6481671
CGCAGAG119400.025.6222748
TACATGG137250.025.1128122
ACATGGG143600.023.0511233
GTGGTAT23250.022.2467961
GCAGAGT135050.021.8554529
GTACTAG15250.020.9670241
CATGGGG115000.020.6063294
GTGTAGC22750.019.0153431
TAGTACT19600.018.4715164
ATGGGGG76650.018.4012415
AGAGTAC120300.018.26575310-11
Produced by FastQC (version 0.11.5)