FastQCFastQC Report
Thu 26 May 2016
ERR580275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639250
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.11290787937074946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.043.1653561
GTATCAA78150.039.4693371
CAACGCA93650.032.1667445
ATCAACG94100.031.963863
TATCAAC100800.030.444972
TCAACGC99650.030.2771224
AACGCAG104000.029.7789236
ACGCAGA117500.025.797577
CGCAGAG123050.024.4430438
GTACATG137200.023.4788951
TACATGG138400.022.9208432
GTACTAG15450.021.9793641
ACATGGG141800.021.5761343
GCAGAGT141300.020.82049
GTGGTAT24850.019.928471
CATGGGG112650.019.899594
CGTTAAC5250.019.7639831
AGAGTAC122050.018.94428610-11
TATCACG3004.2509782E-918.7984852
CAGAGTA132900.017.82200210-11
Produced by FastQC (version 0.11.5)