FastQCFastQC Report
Thu 26 May 2016
ERR580274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139414
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144650.2814523212179443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112990.21984996733090584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72990.14202008244519707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72800.050.073511
GTATCAA136500.042.0746731
GTACTAG31100.035.0885431
CAACGCA156900.034.6484455
ATCAACG160250.033.9017143
TATCAAC173950.032.314242
AACGCAG177000.031.3340556
GTACATG207700.030.9124831
TCAACGC180500.030.435034
TACATGG211800.029.8041672
ACATGGG209000.028.8522383
ACGCAGA194300.028.084817
GCATCGT33950.027.825778
CGCAGAG200350.027.1884868
CATGGGG158200.026.7574164
CTAGGCA47000.026.4188654
ATGGGGG119350.026.281635
TAGGCAT45150.026.0394135
TACCTGG30200.025.855422
CTGTGCG45900.024.575979
Produced by FastQC (version 0.11.5)