FastQCFastQC Report
Thu 26 May 2016
ERR580274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139414
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131090.255067990241689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102960.20033412369581433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72710.1414752732509971No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75150.049.04081
GTATCAA136900.039.590991
CAACGCA159850.032.6362725
ATCAACG163900.031.8298193
TATCAAC177350.030.2380032
AACGCAG180350.029.7077986
GTACATG206500.029.525031
TACCTGG30050.029.0915642
GTACTAG32700.029.0384921
TCAACGC184700.028.4234284
TACATGG210650.028.3584632
ACATGGG207250.027.8253213
CTGTGCG45100.026.3648769
ACGCAGA201100.026.0582057
CGCAGAG205850.025.4797448
CATGGGG155800.025.2793984
TAGTACT47350.024.616254
TAGGCAT44750.024.3660495
GGGCACC44600.024.24219194
ATGGGGG116400.023.9841125
Produced by FastQC (version 0.11.5)