FastQCFastQC Report
Thu 26 May 2016
ERR580273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5640820
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188930.3349335734875426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157610.2794097312092923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89660.15894852166883539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103500.053.48051
GTATCAA186500.040.245141
CAACGCA214100.033.7683685
ATCAACG215850.033.5584373
TATCAAC231750.032.093632
GTGGTAT34950.031.0830881
AACGCAG237500.031.0416496
TCAACGC241400.030.1636964
GTACTAG38750.029.734031
ACGCAGA251500.028.6044887
CGCAGAG254700.028.1703158
GCATCGT38100.027.1318428
TAGGCAT48550.025.85195
GCAGAGT278050.025.367299
GTACATG222850.025.154921
TACATGG223650.024.6792132
CTGTGCG43750.024.2742969
ACATGGG221200.023.9066413
CTAGGCA53000.023.8587044
TGGTATC43800.023.6146072
Produced by FastQC (version 0.11.5)